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A metaproteomic platform for integrated host-pathogen-microbiota profiling in zebrafish larvae.

Javiera Ortiz-Severín1, Antonia Ramos-Guzmán1, Ian Pérez1

  • 1Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile. fpchavez@uchile.cl.

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Summary

This study introduces a metaproteomic workflow to analyze host, pathogen, and microbiota interactions simultaneously. This method provides integrated insights into complex infection systems, advancing our understanding of host-pathogen-microbiota dynamics.

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Area of Science:

  • Microbiology
  • Proteomics
  • Systems Biology

Background:

  • Host-pathogen-microbiota interactions are complex and require multi-compartment analytical strategies.
  • Current methods may not fully resolve all biological components within a single framework.

Purpose of the Study:

  • To develop and validate a global metaproteomic workflow for simultaneous profiling of host, pathogen, and microbiota.
  • To demonstrate the utility of this approach in a zebrafish infection model.

Main Methods:

  • Development of a peptide-centric metaproteomic workflow.
  • Application to zebrafish larvae infected with *Pseudomonas aeruginosa*.
  • Integrated analysis of taxonomic and functional data across all system components.

Main Results:

  • The metaproteomic workflow successfully profiled host, pathogen, and microbiota simultaneously.
  • Identified route-dependent differences in microbial composition and function.
  • Revealed concurrent host proteome remodeling during infection.

Conclusions:

  • Metaproteomics offers a versatile platform for dissecting complex host-pathogen-microbiota systems *in vivo*.
  • This unified proteomic framework enables comprehensive study of infection-driven biological networks.