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Published on: March 29, 2015
A compact visible-light spectrometric detector for capillary electrophoresis
Veiko Rütter1, Mihkel Kaljurand2, Jelena Gorbatsova2
1Division of Gene Technology and Biomedicine, Department of Chemistry and Biotechnology, Faculty of Science, Tallinn University of Technology, Tallinn, Estonia; Research Laboratory for Cognitronics, Thomas Johann Seebeck Department of Electronics, School of Information Technologies, Tallinn University of Technology, Tallinn, Estonia.
Abstract:
This article presents a compact and portable capillary electrophoresis (CE) detector that uses a light-emitting diode (LED) for excitation and a Hamamatsu C12880MA mini spectrometer for detecting substances that absorb visible light. The detector features a robust design, including an adjustable slit that allows for precise optical alignment with the capillary detection window, thereby reducing interference from scattered LED light. All parts are housed within a custom 3D-printed enclosure. Unlike standard portable CE detectors, this device captures full absorbance spectra for better compound identification. The custom instrument separated food colorants with dye detection limits near 40 μg/mL. Results were linear from 20-1000 μg/mL, with correlation coefficients over 0.999 and peak height RSDs of 5-8%.
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