Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

2.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.2K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.4K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterizing pancreatic cancer-associated fibroblast heterogeneity in vascular microphysiological systems.

Angiogenesis·2026
Same author

Not Attending ARVO? A Missed Opportunity.

Veterinary ophthalmology·2026
Same author

Coaxially Electrospun Myocardial dECM- Based Nanofibrous Scaffolds Demonstrate Enhanced Cardiomyocyte Biocompatibility.

Advanced healthcare materials·2026
Same author

Novel green synthesis of polyfunctionally substituted phthalazines promoted by visible light, DFT studies and molecular docking with antimicrobial and antibiofilm potency.

Scientific reports·2026
Same author

Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.

Chemical & biomedical imaging·2026
Same author

Dedifferentiated Adipocytes Improve Heart Function Post-Myocardial Infarction.

Journal of regenerative medicine·2026
Same journal

Cryogel-based therapeutic platforms for disease modification in osteoarthritis.

npj biomedical innovations·2026
Same journal

Ancestry-linked IL-10 signaling and macrophage activation modulate fibroblast responses to oxidative stress in a PEG-based microphysiological system.

npj biomedical innovations·2026
Same journal

In vitro modeling of inflammatory bowel diseases using a newly developed immunocompetent colon epithelial monolayer co-culture model.

npj biomedical innovations·2026
Same journal

Focused ultrasound-induced cavitation in a human brain microphysiological system produces injury signaling biomarkers consistent with blast trauma.

npj biomedical innovations·2026
Same journal

Human colon organoid Transwell model for investigating epithelial growth and repair.

npj biomedical innovations·2026
Same journal

Development and early feasibility testing of machine-learning algorithms to non-invasively assess hemoglobin levels.

npj biomedical innovations·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.6K

Surface modified electrospun scaffold supports iPSC-derived limbal stem cell function.

Nasif Mahmood1, Daxian Zha1, Sarah Gullion1

  • 1Department of Textile Engineering, Chemistry and Science, Wilson College of Textiles, North Carolina State University, Raleigh, NC, USA.

Npj Biomedical Innovations
|April 24, 2026
PubMed
Summary
This summary is machine-generated.

This study developed a functionalized Poly Lactide-co-Glycolic Acid (PLGA) scaffold to regenerate the ocular surface for Limbal Stem Cell Deficiency (LSCD) patients using stem cells.

More Related Videos

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

7.2K
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

13.0K

Related Experiment Videos

Last Updated: Apr 26, 2026

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.6K
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

7.2K
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

13.0K

Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Stem Cell Biology

Background:

  • Limbal Stem Cell Deficiency (LSCD) impairs ocular surface regeneration.
  • Poly Lactide-co-Glycolic Acid (PLGA) is a common biomaterial but requires surface modification for cell support.
  • Induced pluripotent stem cell-derived limbal stem cells (iPSC-LSCs) offer a potential treatment for LSCD.

Purpose of the Study:

  • To develop and characterize an electrospun PLGA scaffold for ocular surface regeneration.
  • To functionalize the PLGA scaffold with extracellular matrix proteins to enhance iPSC-LSC adhesion and survival.
  • To evaluate the scaffold's potential for supporting iPSC-LSC transplantation in LSCD treatment.

Main Methods:

  • Electrospinning of PLGA to create a scaffold structure.
  • Atmospheric plasma treatment for surface functionalization with Collagen IV and Laminin-521.
  • Laser cutting to create micro-perforations for improved transparency and permeability.
  • Assessment of iPSC-LSC attachment, proliferation, and marker expression on the functionalized scaffold.

Main Results:

  • Laminin-521 functionalization was critical for iPSC-LSC attachment and survival.
  • The scaffold supported iPSC-LSC proliferation and maintenance of stemness markers.
  • Enhanced expression of corneal epithelial differentiation markers was observed on the functionalized scaffold.
  • Micro-perforations improved scaffold transparency and permeability.

Conclusions:

  • The developed PLGA scaffold, functionalized with Laminin-521, shows significant potential for ocular surface regeneration.
  • This scaffold provides a supportive microenvironment for iPSC-LSCs, crucial for LSCD treatment.
  • Further in vivo studies are warranted to confirm therapeutic efficacy for Limbal Stem Cell Deficiency.