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A Gold Nanoparticle-based Aptasensor for Specific Detection of CA125
Ghasem Ebrahimi1,2, Parvin Samadi Pakchin1, Maryam Mousivand3
1Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Galen Medical Journal
|April 27, 2026
Summary
A new aptamer-based biosensor detects ovarian cancer marker CA125 using gold nanoparticle aggregation. This simple, rapid, and cost-effective method shows promise for early cancer detection.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Ovarian cancer detection relies on tumor markers like carbohydrate antigen 125 (CA125).
- Existing detection methods can be complex and costly.
- Development of sensitive and accessible biosensors is crucial for early diagnosis.
Purpose of the Study:
- To develop a novel aptamer-based biosensor for detecting carbohydrate antigen 125 (CA125).
- To utilize the salt-induced gold nanoparticle (AuNP) aggregation phenomenon for biosensing.
- To create a simple, rapid, and cost-effective diagnostic tool for ovarian cancer.
Main Methods:
- Citrate-coated gold nanoparticles (AuNPs) were employed.
- Aptamers were adsorbed onto AuNPs to prevent salt-induced aggregation.
- CA125 binding induced aptamer desorption, leading to AuNP aggregation and spectral changes.
Main Results:
- The aptasensor demonstrated a linear detection range of 15-160 U/mL for CA125.
- A limit of detection (LOD) of 14.41 U/mL was achieved under optimal conditions.
- The biosensor showed good repeatability and selectivity for CA125, even in human serum.
Conclusions:
- The developed aptasensor offers a promising approach for CA125 detection.
- The biosensor exhibits comparable or superior performance to ELISA in terms of selectivity and simplicity.
- This engineered aptasensor represents a valuable tool for early and efficient ovarian cancer diagnosis.
