Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prenatal lipopolysaccharide exposure programs the early-life lung development by modulating mesenchymal fibroblasts in a time- and dose-dependent manner.

BMC medicine·2025
Same author

H<sub>2</sub>O<sub>2</sub> Sensitivity of K<sub>v</sub> Channels in Hypoxic Pulmonary Vasoconstriction: Experimental Conditions Matter.

International journal of molecular sciences·2025
Same author

Piezo1 in PASMCs: Critical for Hypoxia-Induced Pulmonary Hypertension Development.

Circulation research·2025
Same author

Early career member highlights from the 22nd ERS Lung Science Conference: development of chronic lung diseases - from life-spanning mechanisms to preventive therapy.

ERJ open research·2024
Same author

Use of FRET-Sensor 'Mermaid' to Detect Subtle Changes in Membrane Potential of Primary Mouse PASMCs.

Cells·2024
Same author

C-type natriuretic peptide/cGMP/FoxO3 signaling attenuates hyperproliferation of pericytes from patients with pulmonary arterial hypertension.

Communications biology·2024

Related Experiment Video

Updated: Apr 28, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K

Spatial Protein Expression Analysis in Lungs Using Capillary-Based Immunoassay After Laser-Assisted Microdissection.

Stefan Hadzic1, Marija Gredic1, Vanessa Nebel1

  • 1Excellence Cluster Cardio-Pulmonary Institute (CPI), Institute for Lung Health (ILH), Universities of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Justus Liebig University Giessen, Aulweg 130, 35392 Giessen, Germany.

Cells
|April 27, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel workflow combining laser-assisted microdissection and capillary electrophoretic immunoassay for spatial protein analysis in lung tissue. This method enables sensitive detection of low-expressed proteins and validates omics findings in lung injury models.

Keywords:
lung injury and repairprotein expression analysisspatial biology

More Related Videos

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

5.8K
Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs
10:00

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Published on: January 3, 2025

2.9K

Related Experiment Videos

Last Updated: Apr 28, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.8K
Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

5.8K
Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs
10:00

Author Spotlight: Advances in Quantifying Microvascular Density in Aging Murine Lungs

Published on: January 3, 2025

2.9K

Area of Science:

  • Pulmonary Medicine
  • Molecular Biology
  • Proteomics

Background:

  • Understanding lung injury and repair necessitates spatial context.
  • Protein-level validation of transcriptomic data, especially for low-expressed proteins or small samples, remains a challenge.

Purpose of the Study:

  • To present a workflow for spatial protein analysis in lung tissue.
  • To enable sensitive detection of low-expressed proteins and validate omics findings.
  • To investigate cigarette smoke-induced lung injury at the protein level with spatial resolution.

Main Methods:

  • Integration of laser-assisted microdissection (LMD) with capillary electrophoretic immunoassay (CEI).
  • Application of the LMD-CEI workflow to a pre-clinical model of cigarette smoke (CS)-induced lung injury.
  • Orthogonal validation of transcriptome analysis results at the protein level.

Main Results:

  • Demonstrated a sensitive method for spatial protein analysis of small samples or low-expressed proteins.
  • Validated transcriptome findings in CS-induced lung injury, showing endothelial cell marker downregulation and MAPK pathway activation in pulmonary vasculature.
  • Identified spatial capillary rarefaction in LMD-enriched alveolar regions.

Conclusions:

  • The LMD-CEI workflow offers a simple, accessible, and sensitive approach for spatial protein analysis in the lung.
  • This method provides orthogonal validation for omics-based studies.
  • The workflow facilitates deeper understanding of complex lung disease mechanisms and identification of therapeutic targets.