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Evaluation of Post-Processing Time's Influence on Biocompatibility of 3D-Printed Denture Base Resins
Miruna Dinescu1, Vlad Gabriel Vasilescu2, Lucian Toma Ciocan2
1Department of Prosthodontics, Faculty of Dentistry, "Carol Davila" University of Medicine and Pharmacy, 37 Dionisie Lupu Street, District 2, 020021 Bucharest, Romania.
Journal of Functional Biomaterials
|April 27, 2026
Summary
Post-curing duration impacts 3D-printed denture resins' biological performance. Extended polymerization improved V-Print dentbase resin's cell viability and reduced apoptosis, highlighting the importance of 3D printing parameters beyond residual monomers.
Area of Science:
- Biomaterials Science
- Dental Materials
- Cellular Biology
Background:
- Additive manufacturing and light-sensitive resins are advancing.
- The biological performance of 3D-printed resins depends on photopolymerization and post-processing.
- Evaluating resin cytotoxicity requires considering more than just soluble substances.
Purpose of the Study:
- To assess the effect of post-curing duration on cellular response to two denture base resins.
- To compare direct contact and indirect eluate-based cytotoxicity testing methods.
- To understand the influence of 3D printing parameters on resin biocompatibility.
Main Methods:
- Human gingival fibroblasts were exposed to two denture base resins (V-Print dentbase, FotoDent Denture) with varying post-curing durations.
- Evaluated cellular responses included viability, membrane integrity, nitric oxide production, live/dead staining, and caspase-3/7 activity.
- Both direct contact and indirect eluate-based testing pathways were employed.
Main Results:
- Direct contact with resin disks reduced metabolic activity, indicating cellular stress.
- Extended polymerization improved metabolic activity and reduced apoptosis for V-Print dentbase resin.
- FotoDent Denture showed a less uniform cellular response to extended polymerization.
Conclusions:
- Post-curing duration significantly influences the biocompatibility of 3D-printed denture base resins.
- Assessing cytotoxicity solely based on saliva-soluble substances is insufficient; 3D printing parameters are crucial.
- V-Print dentbase resin demonstrated better adaptability to extended polymerization compared to FotoDent Denture.
