Structure-Based Design and Mechanistic Insight for Enhanced Catalytic Activity of Aldo/Keto Reductase AKR13B3 from...

Trichothecene mycotoxins, especially T-2 toxin, represent a significant threat to food safety and public health. Although the enzymatic degradation of deoxynivalenol has been extensively investigated, there are few reports of enzymes capable of efficiently degrading T-2 toxin. This study identified that the aldo-keto reductase AKR13B3 from Devosia A6-243 exhibits 3-keto-DON-degrading and a little T-2 toxin-degrading activity. To address this limitation, a rational design strategy targeting the substrate-binding pocket was employed to enhance its activity. Utilizing site-directed and combinatorial mutagenesis, a double mutant R134F/D217A was successfully screened. R134F/D217A retains catalytic activity towards 3-keto-DON while significantly enhancing its catalytic capacity for T-2. Specifically, the R134F/D217A variant exhibited a 2.88-fold increase in catalytic activity and a 3.15-fold enhancement in catalytic efficiency (k_cat/K_m) relative to the wild type enzyme. Notably, a substantial improvement in thermal stability was also observed. After incubation at 55 °C, the residual activity of the R134F/D217A mutant was 2.63 times that of the wild type. Molecular dynamics (MD) simulations and three-dimensional structural modeling suggested the mechanistic basis for the enhanced performance of the R134F/D217A double mutant. Catalytic enhancement stems from a shortened nucleophilic attack distance, a positively biased electrostatic environment, combined with an enlarged pocket and reduced binding free energy. Concurrently, the increased thermal stability results from decreased flexibility and a more rigid structural architecture. This work presents the first report of AKR13B3 as an effective enzyme for T-2 toxin transformation, and its catalytic activity was significantly enhanced through rational design. Thus, a novel enzymatic strategy was proposed, and could inform future approaches to study issues related to T-2 toxin contamination.