Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme Kinetics

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

Catalytically Perfect Enzymes

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biological Effects of Selenium-Containing Ionic Liquids: Cytotoxicity, Mercury Interaction, and Antiproliferative Activity in Glioblastoma Cells.

Journal of applied toxicology : JAT·2026

Same author

Mechanisms of nicotine toxicity and addiction-like behavior in Drosophila melanogaster: The role of α7 & β2 nicotinic acetylcholine receptor subunits in sensory valence and cellular stress.

Neurotoxicology·2026

Same author

Diphenyl Diselenide and Temozolomide: Downregulation of Inflammatory, Redox, and Tumor-Associated Pathways in Glioblastoma.

Biological trace element research·2026

Same author

E-cigarette exposure impairs associative memory: role of α7 and β2 nicotinic acetylcholine receptors.

Journal of neurogenetics·2026

Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Published on: April 4, 2014

A microvolume protocol for catalase activity.

Beatriz Corrêa da Rocha¹, Marcos Trindade da Rosa Rosa¹, João Batista Teixeira da Rocha²

¹Center for Natural and Exact Sciences, Doctoral Program Toxicological Biochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.

Toxicology Mechanisms and Methods

|April 27, 2026

Summary

A new microvolume protocol accurately measures catalase (CAT) activity using only 2 µL of sample. This efficient method is ideal for limited biological materials and studying oxidative stress responses.

Keywords:

CAT Catalase activity protocol NanoDrop microvolume nano-spectrophotometer oxidative stress

More Related Videos

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

Related Experiment Videos

Last Updated: Jul 5, 2026

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Published on: April 4, 2014

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

Area of Science:

Biochemistry
Enzymology
Spectrophotometry

Background:

Catalase (CAT) activity assays typically require large sample volumes.
Limited biological materials, such as small organisms or specific tissues, pose challenges for traditional enzymatic assays.
Developing microvolume methods is crucial for expanding research capabilities.

Purpose of the Study:

To develop and validate a microvolume protocol for quantifying catalase (CAT) activity.
To enable reliable CAT activity measurements using minimal sample volumes (2 µL).
To provide an efficient alternative for studies involving limited biological material.

Main Methods:

Utilized a NanoDrop spectrophotometer for microvolume CAT activity measurements.
Employed CAT inhibitors and protein denaturation controls to ensure assay specificity.
Validated the protocol using small organisms (Panagrellus redivivus, Caenorhabditis elegans) and Drosophila tissues.

Main Results:

The developed protocol accurately quantifies CAT activity in microvolumes.
Results correlate with the physiological and metabolic status of analyzed tissues.
The method demonstrated high specificity through control experiments.

Conclusions:

This microvolume protocol offers an efficient and reliable method for CAT activity assessment.
It significantly expands experimental possibilities for reduced biological models.
The methodology facilitates investigations into oxidative stress responses in limited sample contexts.