Cold-plasma for the decontamination of laparoscopic trocar incisions: In Vitro antimicrobial efficacy of a prototype...

The antimicrobial efficacy and operating parameters of a cold plasma prototype were investigated. The device concept is intended for use on laparoscopic trocar incisions.

The device (50 mm length, 10 mm diameter, glass-ceramic body with two wire-wound electrodes) was operated with two gas compositions and in different environments. In vitro decontamination was tested on wet agar plates inoculated with Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) as well as on inoculated stainless steel and polypropylene strips. A 5-min plasma exposure was applied.

Both microorganisms were effectively inactivated on wet agar surfaces (inhibition zone assay: up to 36 mm) using different process gas compositions. Additionally, antimicrobial action was confirmed for S. aureus on stainless steel and polypropylene substrates. The prototype thus shows consistent decontamination performance across tested modes.

The plasma source offers a promising, minimally invasive adjunct for preventing surgical site infections during laparoscopic procedures. Further development, advanced biological models, and compliance with regulatory standards (e.g., DIN SPEC 91315) are required before clinical implementation.