Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

On-Chip Raman Spectroscopy for Rapid Antimicrobial Susceptibility Testing from Blood Cultures.

Analytical chemistry·2026
Same author

From bulk samples to single cells: measurement strategies in Raman-based antibiotic susceptibility testing.

Analytical and bioanalytical chemistry·2026
Same author

Current trends in machine learning for surface-enhanced Raman spectroscopy.

The Analyst·2026
Same author

Soft-Matter Confinement Modulates Excited-State Dynamics of Ru Photocatalysts for Hydrogen Evolution in Aqueous Media.

ACS materials Au·2026
Same author

Correction to "Dual-Defect Synergic Cr<sub><i>x</i></sub>Ti<sub><i>y</i></sub>O<sub>2</sub> Nanostructures Boosting High SERS Performance".

The journal of physical chemistry letters·2026
Same author

Photoresponsive Supramolecular Soft Actuators via Co-assembly of Azobenzene Bola-Amphiphiles: From Nanostructure Transformations to Enhanced Actuation Properties.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Formation of Bimetallic Nanoparticles via Exsolution Using a Reducible Metal Oxide Capping Layer.

ACS nano·2026
Same journal

Cold-Driven Thermoelectric Patch for Postoperative Tumor Control.

ACS nano·2026
Same journal

Chemically Fueled Interfacial Supramolecular Polymerization.

ACS nano·2026
Same journal

Tactile Neuromorphic Ion-Gated Vertical Transistor Displays Enabling Dual-Output Reservoir Computing.

ACS nano·2026
Same journal

In Situ Oxygen Shuttling within a Bilayer Electrified Membrane Enables Aeration-Free Electro-Fenton Water Purification.

ACS nano·2026
Same journal

Single Atoms as Growth Directors: From Graphene Edges to Atomically Precise Interfaces in 2D Materials.

ACS nano·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Bioanalysis Using Surface-Enhanced Raman Spectroscopy: From the Perspective of Single Molecules.

Xiaobin Yao1, Jürgen Popp2,3, Jinqing Huang1

  • 1Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China.

ACS Nano
|April 28, 2026
PubMed
Summary
This summary is machine-generated.

Single-molecule surface-enhanced Raman spectroscopy (SM-SERS) offers high sensitivity for biomolecule analysis but faces challenges. This perspective critically examines SM-SERS limitations, including signal fluctuations and data analysis, to improve future research.

Keywords:
bioanalysisquantitative analysissample degradationsignal intensity fluctuationsingle-moleculespatial resolutionsurface-enhanced Raman spectroscopy

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

24.5K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

24.5K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.5K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysics

Background:

  • Single-molecule surface-enhanced Raman spectroscopy (SM-SERS) provides unparalleled sensitivity and specificity for probing biomolecular physicochemical properties.
  • SM-SERS typically involves complex methodologies, rationally designed substrates, and specialized single-molecule techniques, presenting significant technical hurdles.

Purpose of the Study:

  • To critically assess the intrinsic limitations of SM-SERS, moving beyond a comprehensive review.
  • To provide a conceptual framework for advancing the fundamental understanding and practical application of SM-SERS.

Main Methods:

  • Selective overview of representative SM-SERS methods and features.
  • Detailed discussion of overlooked limitations in SM-SERS.

Main Results:

  • Identified persistent issues of reproducibility and reliability in SM-SERS.
  • Highlighted signal intensity fluctuations (SIFs) and sample degradation as key challenges.
  • Addressed the emerging difficulty of big data processing and analysis in SM-SERS.

Conclusions:

  • SM-SERS, despite its potential, requires addressing inherent limitations for reliable application.
  • Overcoming challenges in signal stability, sample integrity, and data analysis is crucial for advancing SM-SERS.
  • A conceptual framework is proposed to guide future research and enhance the practical utility of SM-SERS.