Reproducible Quantification of Pseudomonas aeruginosa via MIP-Functionalized SPR-FPI Optical Fiber Sensor

Area of Science:

• Biomedical Engineering
• Environmental Science
• Analytical Chemistry

Background:

• Accurate detection of Pseudomonas aeruginosa is crucial for marine environmental monitoring.
• Traditional sensors face challenges with repeatability, detection limits, and temperature interference.

Purpose of the Study:

• To develop a reusable optical fiber surface plasmon resonance (SPR) sensor for quantitative detection of Pseudomonas aeruginosa.
• To enhance sensor performance through molecular imprinting and integrate a Fabry-Perot interferometer (FPI) for temperature compensation.

Main Methods:

• Constructing a molecularly imprinted nanofilm on the SPR sensor surface using aptamer DNA and polydopamine.
• Utilizing molecular imprinting to create specific cavities for Pseudomonas aeruginosa.
• Integrating an FPI to quantify and compensate for temperature variations.

Main Results:

• Enhanced SPR sensors showed a significant increase in resonance wavelength response (10.677 nm to 24.98 nm).
• Response time was reduced from 22 minutes to 16 minutes.
• Detection limit improved from 0.00985 OD to 0.00105 OD.
• FPI integration effectively compensated for temperature fluctuations.

Conclusions:

• The developed sensor offers a novel, reliable, and cost-effective solution for marine microbial monitoring.
• Molecular imprinting significantly boosts sensor sensitivity and specificity.
• The integrated FPI enhances environmental adaptability and measurement accuracy.