Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

8.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
8.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

3.0K
3.0K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.5K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

3.0K
3.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

2.3K
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to: "Hypoxia-Induced Lineage Plasticity in Neuroblastoma: Advancing Signature Interpretation and Clinical Translation".

Clinical and translational science·2026
Same author

Maternal trans-vaccenic acid shapes neonatal T cell development and early-life immune imprinting.

Science (New York, N.Y.)·2026
Same author

Spatially resolved m<sup>6</sup>A profiling using m<sup>6</sup>A-ARTR-DBiT.

Nature methods·2026
Same author

CDS-localized m<sup>6</sup>A drives co-translational RNA decay to relieve biotic and abiotic endoplasmic reticulum stresses.

Nature plants·2026
Same author

mRNA m<sup>6</sup>A modifications and the RNA-binding protein YTHDF1 affect translational control in both normal and pathological learning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modifications.

Nature communications·2026

Related Experiment Video

Updated: Apr 30, 2026

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.1K

Regulatory elements on chromatin-associated RNA 15 years beyond RNA epigenetics.

Xiaoyang Dou1, Chuan He2,3

  • 1State Key Laboratory of RNA Innovation, Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. xiaoyang.dou@sibcb.ac.cn.

Nature Chemical Biology
|April 28, 2026
PubMed
Summary

RNA chemical modifications, like N6-methyladenosine, regulate gene expression and offer therapeutic potential. Emerging research shows these modifications on chromatin-associated RNAs (caRNAs) also impact epigenetic regulation.

More Related Videos

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

8.5K
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
08:08

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Published on: April 2, 2018

12.9K

Related Experiment Videos

Last Updated: Apr 30, 2026

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.1K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

8.5K
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
08:08

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Published on: April 2, 2018

12.9K

Area of Science:

  • Epigenetics and RNA Biology

Background:

  • Chemical modifications on RNA, such as N6-methyladenosine (m6A), are crucial regulators of gene expression, analogous to DNA and histone epigenetic marks.
  • Extensive characterization of mRNA modifications has revealed their roles in RNA metabolism and their implications for physiological and pathological processes.
  • These discoveries pave the way for novel therapeutic strategies targeting RNA modification pathways.

Purpose of the Study:

  • To summarize established principles of post-transcriptional RNA modifications and their therapeutic potential.
  • To highlight the emerging roles of RNA modifications on chromatin-associated RNAs (caRNAs) in epigenetic regulation.
  • To discuss future research directions for understanding RNA modifications in gene expression.

Main Methods:

  • Literature review and synthesis of established principles in RNA modification biology.
  • Analysis of emerging evidence on the intersection of RNA methylation and chromatin regulation.
  • Discussion of therapeutic potential and future research avenues.

Main Results:

  • N6-methyladenosine (m6A) and other mRNA modifications are well-characterized regulators of RNA metabolism with therapeutic implications.
  • RNA methylation on chromatin-associated RNAs (caRNAs) is increasingly recognized as a key player in modulating chromatin state and transcription.
  • RNA modifications on caRNAs introduce a new layer of epigenetic regulation with significant biological implications.

Conclusions:

  • Post-transcriptional RNA modifications are fundamental to gene expression regulation and hold significant therapeutic promise.
  • RNA modifications on caRNAs represent a novel frontier in epigenetic regulation, influencing chromatin state and transcription.
  • Further investigation into regulatory elements on caRNAs is essential for a comprehensive understanding of gene expression programs.