Drift-Compensated Real-Time Electronic Sensing Platform for Cardiac Markers' Intelligent Multiplex Detection in Whole...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• Accurate early diagnosis of acute myocardial infarction (AMI) is crucial but hindered by complex, lab-based conventional methods.
• Point-of-care testing (POCT) requires rapid, sensitive, and user-friendly diagnostic tools for decentralized applications like home use.

Purpose of the Study:

• To develop an electrochemical sensing integrated chip (Sensing IC) for rapid, sensitive, and accurate detection of cardiac markers.
• To create a smartphone-compatible system for home-based, real-time diagnosis of AMI.

Main Methods:

• An electrochemical Sensing IC utilizing AuNPs/MXene electrodes with a dual-signal drift-correction mechanism and dual-reporter system was developed.
• The Sensing IC was coupled with a smartphone-based portable analyzer for multiplex detection in whole blood.
• Dual redox reporters were employed to minimize signal drift and enable in situ calibration.

Main Results:

• The Sensing IC achieved picomolar limit of detection, demonstrating exceptional sensitivity.
• The smartphone-based system enabled real-time, high-sensitivity multiplex detection of cardiac markers directly in whole blood.
• The developed POCT system showed comparable accuracy to standard assays, reducing detection time by ~90% and simplifying sample preprocessing.

Conclusions:

• The developed Sensing IC platform facilitates early AMI diagnosis and timely clinical warnings.
• This intelligent, home-based cardiovascular monitoring system meets the demand for rapid, decentralized diagnostics.
• The technology holds significant potential for improving patient outcomes through accessible and efficient cardiac marker detection.