Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
The Nucleolus02:55

The Nucleolus

8.6K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

4.4K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

7.6K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.6K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solitary intracranial metastasis as the sole relapse site in gastric cancer patients after neoadjuvant therapy and surgery: a case series of 12 patients and clinical implications.

Frontiers in oncology·2026
Same author

SMPD1 modulates malignant progress of osteosarcoma through ferroptosis pathway.

Tissue & cell·2026
Same author

Aptamer-mediated portable SERS sensor based on bimetallic magnetic nanotubes for internal standard-calibrated detection of multi-target disease markers.

Biosensors & bioelectronics·2026
Same author

The vesicle transport gene SEC23A is a novel prognostic indicator and therapeutic target in gastric cancer.

Frontiers in oncology·2026
Same author

DLST mediates the malignant progression of osteosarcoma cells by regulating the p38 MAPK signaling pathway.

Biochemical and biophysical research communications·2026
Same author

Polystyrene Nanoparticles Cause Sex-Specific Toxicity in Male Zebrafish, Which Can Be Mitigated by Melatonin.

Environmental toxicology·2026

Related Experiment Video

Updated: May 1, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

5.3K

E3 ubiquitin ligase RNF40: Structure, function and its context‑dependent roles in tumorigenesis (Review).

Zikun Wu1, Hang Yang1, Guanglong Chen1

  • 1Department of General Surgery, The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, Henan 450008, P.R. China.

Oncology Reports
|April 30, 2026
PubMed
Summary
This summary is machine-generated.

The ubiquitin-proteasome system is crucial for cell health and disease. Ring finger protein 40 (RNF40) is a key regulator in this system, impacting cancer and neurodegenerative diseases.

Keywords:
E3 ubiquitin ligaseRNF40cancerubiquitin

More Related Videos

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

10.3K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Related Experiment Videos

Last Updated: May 1, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

5.3K
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

10.3K
Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates
09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

2.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • The ubiquitin-proteasome system (UPS) is a vital cellular machinery for protein degradation, regulating fundamental biological processes.
  • Dysregulation of the UPS is implicated in various diseases, including cancers and neurodegenerative disorders like Parkinson's disease.

Purpose of the Study:

  • To systematically review the structural architecture of ring finger protein 40 (RNF40).
  • To elucidate the multifaceted roles of RNF40 in ubiquitination-dependent proteostasis, epigenetic modulation, and DNA repair.
  • To explore RNF40's tumor-specific regulatory networks and its potential as a therapeutic target in cancer.

Main Methods:

  • Literature review of existing studies on RNF40.
  • Analysis of RNF40's structural features and functional domains.
  • Examination of RNF40's involvement in various cellular pathways and disease models.

Main Results:

  • RNF40 is identified as a key E3 ubiquitin ligase regulating proteostasis.
  • RNF40 plays significant roles in epigenetic modifications and DNA damage repair mechanisms.
  • RNF40 exhibits distinct regulatory networks across different cancer subtypes, influencing disease progression.

Conclusions:

  • RNF40 is a critical regulator of cellular homeostasis with implications in disease pathogenesis.
  • Understanding RNF40's functions offers insights into cancer biology and potential therapeutic strategies.
  • RNF40 represents a promising novel drug target for cancer therapy.