Protocol for AI-powered classification of IDH-mutant astrocytoma using GUIDE

Accurate identification of prognostic cancer subtypes remains challenging using routine diagnostics alone. Here, we present a protocol to obtain isocitrate dehydrogenase (IDH)-mutant astrocytoma subtype predictions using the generic utility for immune/mesenchymal-enriched (IME) diagnostic estimation (GUIDE). We describe steps for preparation of image and omics inputs, setup of the GUIDE Docker container, execution of subtype inference, and retrieval of standardized output files. GUIDE integrates histopathology images and multi-omics data and enables robust and flexible subtype classification under variable data availability. For complete details on the use and execution of this protocol, please refer to Tang et al.¹.