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Related Concept Videos

The Phosphorus Cycle01:21

The Phosphorus Cycle

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Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.
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Gravimetry: Inorganic And Organic Precipitating Agents00:49

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In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
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The elemental makeup of a compound defines its chemical identity, and chemical formulas are the most concise way of representing this elemental makeup. When a compound’s formula is unknown, measuring the mass of its constituent elements is often the first step in determining the formula experimentally.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
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Factors Affecting Solubility04:01

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Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
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Related Experiment Video

Updated: May 2, 2026

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
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Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method

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Determining Inorganic and Organic Phosphorus.

Jaana Koistinen1, Mervi Sjöblom2, Kristian Spilling3,4

  • 1Tvärminne Zoological Station, University of Helsinki, Hanko, Finland. jaana.koistinen@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2026
PubMed
Summary

This study introduces a colorimetric method to measure phosphorus (P) in microalgae. The technique quantifies orthophosphate (PO4), dissolved organic phosphorus (DOP), and particulate organic phosphorus (POP) for better nutrient analysis.

Keywords:
OrthophosphateParticulate organic phosphorusTotal phosphorus

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Last Updated: May 2, 2026

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Area of Science:

  • Environmental Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Phosphorus is an essential macronutrient for microalgal growth.
  • Orthophosphate (PO4) is the primary form of phosphorus uptake by microalgae.
  • Accurate measurement of phosphorus forms is crucial for understanding aquatic ecosystems.

Purpose of the Study:

  • To present a colorimetric method for determining orthophosphate (PO4) concentration.
  • To describe a method for quantifying dissolved organic phosphorus (DOP).
  • To outline a method for measuring particulate organic phosphorus (POP).

Main Methods:

  • A colorimetric method based on total phosphorus (TP) measurements.
  • Determination of PO4 concentration using spectrophotometry.
  • Calculation of DOP and POP from TP measurements.

Main Results:

  • The developed method allows for the quantification of PO4, DOP, and POP.
  • The colorimetric approach provides a reliable way to assess phosphorus fractions.
  • The methods are based on established principles of total phosphorus analysis.

Conclusions:

  • The presented colorimetric methods enable comprehensive phosphorus speciation in microalgal samples.
  • This technique aids in understanding phosphorus cycling and bioavailability in aquatic environments.
  • The methods offer a practical approach for researchers studying microalgal nutrition and phosphorus dynamics.