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Updated: May 3, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Optical-sectioned fluorescent imaging by dynamic mask projection and dark-channel dehazing
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Defocused background is the major source degrading the quality of wide-field fluorescence imaging. Here, we report an optical sectioning method based on the characteristics of background fluorescence under two complementary illumination patterns and dark-channel dehazing. The method is physically interpretable, deconvolution-free, and easy to implement on all epi-fluorescence microscopes. Three-dimensional imaging of mitochondria, mouse liver neuronal tissue, and zebrafish blood vessels demonstrates its superior 3D imaging capability for thick samples with high fidelity and a large field of view.
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