Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Obstructive Pulmonary Disease II: Emphysema01:23

Chronic Obstructive Pulmonary Disease II: Emphysema

48
Emphysema, a major phenotype of chronic obstructive pulmonary disease (COPD), is characterized by irreversible destruction of alveolar walls and permanent enlargement of distal airspaces. Unlike chronic bronchitis, which primarily affects the airways, emphysema predominantly involves the lung parenchyma, where structural damage leads to airflow limitation.PathophysiologyIt most commonly results from prolonged exposure to cigarette smoke and other toxic gases, particularly cigarette smoke.
48

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of synthetic vascular organoids via orthogonal programming of human pluripotent stem cells.

Cell regeneration (London, England)·2026
Same author

Causal effects of circulating inflammatory proteins on COPD: A Mendelian randomization study.

Medicine·2026
Same author

CCR5+ CD8+ T Cells Are Associated with Poor Response to PD-1 Blockade Therapy.

International journal of molecular sciences·2026
Same author

Synchronous Surgical Therapy for Bilateral Multiple Pulmonary Nodules: A Single-Center Analysis of 108 Cases.

Thoracic cancer·2026
Same author

Origin, Niche, and State: A Three-Body Problem in Lung Macrophage Biology.

American journal of respiratory and critical care medicine·2026
Same author

Association Between Chronic PM2.5 Exposure and CT-Detected Lung Abnormalities in the SPIROMICS Cohort.

Annals of the American Thoracic Society·2026

Related Experiment Video

Updated: May 3, 2026

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

5.4K

Lung-Targeted HGF mRNA Restores Alveolar Structure in Experimental Emphysema.

Dian Chen1, Jeffrey L Curtis2,3, Weijing Kong4

  • 1Department of Respiratory and Critical Care Medicine, Peking University Third Hospital, Beijing, China.

The European Respiratory Journal
|May 1, 2026
PubMed
Summary

Hepatocyte growth factor (HGF) mRNA therapy delivered via lipid nanoparticles (LNPs) shows promise for treating emphysema. This approach restores lung function and reduces alveolar destruction by activating repair pathways in lung cells.

More Related Videos

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
09:02

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

Published on: June 28, 2018

13.0K
A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis
05:45

A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis

Published on: January 16, 2026

629

Related Experiment Videos

Last Updated: May 3, 2026

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

5.4K
Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
09:02

Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment

Published on: June 28, 2018

13.0K
A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis
05:45

A Refined Aerosol-Based Intratracheal Bleomycin Delivery Method for Reproducible and Minimally Invasive Mouse Models of Pulmonary Fibrosis

Published on: January 16, 2026

629

Area of Science:

  • Regenerative Medicine
  • Pulmonary Medicine
  • Nanotechnology in Therapeutics

Background:

  • Emphysema, a component of COPD, involves progressive alveolar destruction with limited treatment options.
  • Hepatocyte growth factor (HGF) has regenerative potential but faces delivery and expression challenges in the lungs.

Purpose of the Study:

  • To investigate the therapeutic potential of HGF mRNA delivered via lipid nanoparticles (LNPs) for emphysema.
  • To assess the efficacy and mechanisms of HGF mRNA therapy in preclinical emphysema models.

Main Methods:

  • Assessed HGF expression in human emphysema datasets and animal models.
  • Utilized a clinical-stage SM102 LNP platform for HGF mRNA delivery.
  • Evaluated therapeutic efficacy via intratracheal instillation and nebulization in elastase- and cigarette smoke-induced emphysema models.
  • Investigated mechanisms using single-cell RNA sequencing (scRNA-seq) and validated in lung organoids.

Main Results:

  • HGF expression varied with emphysema severity, decreasing in advanced stages.
  • HGF mRNA LNPs improved lung function and reduced alveolar destruction in the elastase model.
  • Nebulized HGF mRNA therapy showed comparable efficacy in the cigarette smoke model, improving lung function, reducing inflammation, and decreasing apoptosis.
  • scRNA-seq revealed enhanced alveolar type II (AT2) cell proliferation and differentiation.

Conclusions:

  • HGF mRNA therapy using LNPs is a viable strategy for emphysema treatment.
  • Both direct instillation and nebulization achieved structural and functional recovery.
  • The therapy activates endogenous repair pathways in AT2 cells, demonstrating proof-of-concept for emphysema treatment.