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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Related Experiment Video

Updated: May 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Latest Developments in Mass Spectrometry-Based Techniques for Metabolomics Analysis.

Marisa Maia1,2, Jasmine Hertzog3, Vincent Carré4

  • 1Grapevine Pathogen Systems Lab (GPS Lab), Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal.

Advances in Experimental Medicine and Biology
|May 3, 2026
PubMed
Summary
This summary is machine-generated.

Metabolomics, a complex OMICs science, studies small molecules (metabolites) using mass spectrometry (MS). This chapter covers targeted and untargeted approaches, MS techniques, and new single-cell and data integration methods.

Keywords:
Ionization sourcesMass analyzersMass spectrometry imagingMetabolomicsTargeted and untargeted analysis

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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Last Updated: May 5, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Area of Science:

  • Metabolomics, a rapidly advancing field within the OMICs sciences.
  • Focuses on the comprehensive study of small molecules (metabolites) within biological systems.

Background:

  • Metabolomics aims to elucidate cellular processes and metabolic networks.
  • Advanced analytical techniques, particularly mass spectrometry (MS), are crucial for metabolite detection, identification, and quantification.

Purpose of the Study:

  • To present the two primary metabolomics approaches: targeted and untargeted.
  • To discuss the advantages, limitations, and complementary strengths of each approach.
  • To review mass spectrometry (MS) techniques, including instruments and ionization sources, for metabolite analysis.

Main Methods:

  • Exploration of targeted metabolomics for hypothesis-driven research.
  • Discussion of untargeted metabolomics for broad metabolite profiling.
  • Overview of various mass spectrometry (MS) instruments and ionization sources.

Main Results:

  • Comparison of the strengths and weaknesses of targeted versus untargeted metabolomics.
  • Analysis of the applicability of different MS techniques based on performance.
  • Highlighting recent advancements in single-cell metabolomics and multi-OMICs data integration.

Conclusions:

  • Metabolomics offers deep insights into biological systems through metabolite analysis.
  • The choice between targeted and untargeted metabolomics depends on research objectives.
  • Emerging techniques like single-cell metabolomics and data integration are expanding the field's capabilities.