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    This study introduces a real-time correction method for stimulated Raman scattering (SRS) microscopy, significantly improving quantitative chemical imaging by compensating for transmission losses and enhancing image uniformity.

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    Area of Science:

    • Optics and Photonics
    • Chemical Imaging
    • Microscopy

    Background:

    • Transmission losses in stimulated Raman scattering (SRS) microscopy introduce artifacts, degrading quantitative chemical imaging.
    • Accurate chemical analysis using SRS requires compensation for signal variations caused by light attenuation.

    Purpose of the Study:

    • To develop and validate a real-time electronic gain control scheme for compensating pump and Stokes transmission losses in SRS microscopy.
    • To improve the quantitative accuracy and uniformity of chemical images obtained via SRS microscopy.

    Main Methods:

    • A real-time correction scheme employing active electronic gain control to independently adjust pump and Stokes signals.
    • Utilizing backscattered pump light to measure pump transmission factors.
    • Exploiting a variable gain amplifier's logarithmic interface for efficient compensation calculations.

    Main Results:

    • The developed method effectively compensates for both pump and Stokes transmission losses.
    • Chemically specific contrast in SRS images becomes largely independent of transmission variations.
    • Significant improvements in image uniformity were achieved for homogeneous and heterogeneous samples.

    Conclusions:

    • The real-time correction scheme represents a significant advancement towards accurate quantitative chemical imaging with SRS microscopy.
    • This technique minimizes artifacts caused by transmission losses, enhancing the reliability of SRS-based chemical analysis.