Related Experiment Video
Updated: May 5, 2026
09:30
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
Published on: January 18, 2017
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Fast wide-field light sheet electro-optic FLIM
Optics Express
|May 4, 2026
Abstract:
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet electro-optic FLIM (LS-EO-FLIM) acquisition with up to 800 μm field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.
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