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Related Experiment Video

Updated: May 5, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Fast wide-field light sheet electro-optic FLIM.

V Rose Knight, Nils Bode, Dara P Dowlatshahi

|May 4, 2026

Summary

This summary is machine-generated.

We developed a new method for volumetric fluorescence lifetime microscopy (FLIM) using electro-optic gating. This light sheet electro-optic FLIM (LS-EO-FLIM) technique enables rapid 3D imaging of biological samples.

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Area of Science:

Biophotonics
Microscopy
Molecular Imaging

Background:

Fluorescence Lifetime Microscopy (FLIM) provides functional and molecular information.
Traditional FLIM methods can be slow for volumetric imaging.
Selective Plane Illumination Microscopy (SPIM) offers optical sectioning for 3D imaging.

Purpose of the Study:

To demonstrate volumetric FLIM using electro-optic gating in a SPIM setup.
To enable faster 3D FLIM acquisition.
To image biological samples with high spatial and temporal resolution.

Main Methods:

Implemented electro-optic FLIM (EO-FLIM) with a Pockels cell.
Integrated EO-FLIM gating with a selective plane illumination microscope (SPIM).
Achieved light sheet electro-optic FLIM (LS-EO-FLIM) with an 80 MHz gating frequency and 800 µm field of view.

Main Results:

Demonstrated successful volumetric imaging of fluorescent bead mixtures.
Acquired 3D FLIM data from live Arabidopsis thaliana root samples.
Utilized both genetically encoded fluorescent proteins and endogenous autofluorescence for imaging.

Conclusions:

LS-EO-FLIM is a viable technique for rapid volumetric fluorescence lifetime imaging.
The method allows for imaging of diverse biological samples.
This technique advances 3D functional imaging in live biological systems.