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Updated: Jul 25, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Studies on the fluorophore sempervirene and its complexes with DNA
Abstract:
Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervirene are bound to DNA, energy can be transferred from sempervirene to ethidium. Sempervirene is taken up by mammalian cells and appears in the cytoplasm. This unusual new probe should be useful in molecular and cellular investigations.
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