Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

3.6K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
3.6K
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

984
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
984
Bioplastics01:27

Bioplastics

70
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
70

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Valorization of artichoke processing by-products via production of oligosaccharide-enriched extracts optimized by artificial neural networks.

International journal of biological macromolecules·2026
Same author

Coupled organic matter degradation and dynamic extracellular polysaccharide turnover by an alkaline microalgae-cyanobacteria consortium treating maize lime cooking wastewater.

Journal of environmental management·2025
Same author

Differences and Similarities Between the Carbohydrates, Lipids, and Phenolic Compounds in Sorghum and Maize Grains Used for Popping, Implications for Human Nutrition and Health.

Plant foods for human nutrition (Dordrecht, Netherlands)·2025
Same author

Exploring the Impact of Olive-Derived Bioactive Components on Gut Microbiota: Implications for Digestive Health.

Foods (Basel, Switzerland)·2025
Same author

Enzymatic synthesis of β-galactosylated xylitol derivatives modulates gut microbiota and improves obesity-related metabolic parameters in mice.

Food & function·2025
Same author

Global Status, Recent Trends, and Knowledge Mapping of Olive Bioactivity Research Through Bibliometric Analysis (2000-2024).

Foods (Basel, Switzerland)·2025

Related Experiment Video

Updated: May 5, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
10:03

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

868

Pectic Polysaccharides Recovery from Rapeseed Meal via Conventional and Enzyme-Assisted Extraction Techniques: Toward

Katarina Banjanac1, Milica Veljković1, Milica Simović2

  • 1Innovation Center of Faculty of Technology and Metallurgy, Karnegijeva 4, 11120 Belgrade, Serbia.

Foods (Basel, Switzerland)
|May 4, 2026
PubMed
Summary

Researchers developed a sustainable method to extract pectic polysaccharides from rapeseed meal (RSM) and convert them into prebiotic pectic oligosaccharides (POS). The resulting POS showed enhanced prebiotic activity, particularly for Lactobacillaceae bacteria.

Keywords:
enzyme-assisted extractionpectic oligosaccharidespectic polysaccharidesprebioticsrapeseed meal

More Related Videos

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.8K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

14.2K

Related Experiment Videos

Last Updated: May 5, 2026

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis
10:03

Comparative Study on the Polysaccharide Contents and Antioxidant Activities of Hippophae rhamnoides subsp. sinensis and Hippophae gyantsensis

Published on: August 15, 2025

868
OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides

Published on: June 20, 2010

13.8K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

14.2K

Area of Science:

  • Biochemistry and Food Science
  • Biotechnology
  • Sustainable Chemistry

Background:

  • Rapeseed meal (RSM) is a rich source of pectic polysaccharides.
  • Developing novel prebiotics from agricultural byproducts aligns with biorefinery principles.
  • Enzyme-assisted extraction offers a greener alternative to conventional methods.

Purpose of the Study:

  • To investigate enzyme-assisted extraction of pectic polysaccharides from RSM.
  • To produce prebiotic pectic oligosaccharides (POS) from extracted fractions.
  • To evaluate the prebiotic potential of the produced POS.

Main Methods:

  • A two-step enzymatic extraction process using Alcalase® and Cellic® CTec3 HS, followed by ammonium oxalate extraction.
  • Compositional analysis of pectic polysaccharide fractions, including galacturonic acid content and domain structure.
  • Enzymatic hydrolysis of pectic fractions using Pectinex® Ultra Passover to produce POS.
  • Assessment of prebiotic activity of POS against specific bacterial strains and yeast.

Main Results:

  • Two pectic polysaccharide fractions (PP-EAE and CaPP-EAE) were successfully isolated.
  • CaPP-EAE was characterized as low-methylated pectin with significant homogalacturonan and rhamnogalacturonan I domains.
  • Enzymatic treatment yielded short-chain POS with demonstrated enhanced prebiotic activity for Lactobacillaceae.

Conclusions:

  • A sustainable, biorefinery-compatible method for RSM polysaccharide extraction and POS production was established.
  • Structurally defined POS derived from RSM exhibit promising prebiotic properties.
  • This approach supports the valorization of agricultural byproducts into functional food ingredients.