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Updated: Aug 29, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
Published on: May 1, 2013
Digestive stability and prebiotic potential of radish green polysaccharide via mucin-associated intestinal barrier
Miri Park1, Wonchan Yoon1, Hee-Jin Kim1,2
1Food Functionality Research Division, Korea Food Research Institute, 245 Nongsaenmyeong-Ro55365, Wanju-Gun, Jeollabuk-Do Republic of Korea.
Abstract:
The gut microbiota and intestinal barrier are critical determinants of metabolic health. Radish greens (Raphanus sativus), an underutilized agricultural byproduct, are a promising source of functional polysaccharides, yet their digestive stability, prebiotic activity, and mucin-associated epithelial responses remain poorly defined. Here, we evaluated the digestive fate and intestinal functional properties of radish green polysaccharides (RGP). Under simulated gastrointestinal digestion, RGP showed partial structural degradation while largely preserving monosaccharide composition, indicating relative digestive stability. Both intact and digested RGP significantly promoted the growth of beneficial gut bacteria, lowered culture pH, and exhibited prebiotic effects comparable to or greater than those of inulin. In mucin-secreting LS174T cells, RGP enhanced mucin production and upregulated mucin-associated genes, whereas digested RGP elicited similar but weaker responses. Transcriptomic profiling further showed selective modulation of epithelial regulatory programs without broad transcriptional disruption. These findings support RGP as a sustainable gut barrier-oriented functional ingredient with retained activity after digestion.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-026-02228-3.
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