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Related Experiment Video

Updated: May 5, 2026

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

Bioinformatics and Preliminary Functional Analysis of OsPP2C61.

Hao Wang¹, Enjie Xu¹, Yujiao Shi¹

¹Tianjin Key Laboratory of Intelligent Breeding of Major Crops, College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China.

|May 4, 2026

Summary

This study characterizes OsPP2C61, a rice protein phosphatase involved in plant stress response and cell elongation. It is highly expressed in roots and localizes to the nucleus and plasma membrane, offering potential for rice improvement.

Keywords:

OsPP2C61 bioinformatics rice (Oryza sativa L.)spatiotemporal expression subcellular localization

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Published on: August 21, 2016

Area of Science:

Plant molecular biology
Plant signaling pathways
Biochemistry

Background:

Protein phosphatase 2Cs (PP2Cs) are crucial for plant signal transduction.
The PP2C.D subfamily regulates cell elongation and stress adaptation via auxin signaling.
OsPP2C61 in rice is a PP2C member with unclear molecular characteristics.

Purpose of the Study:

To conduct a preliminary characterization of the rice OsPP2C61 gene.
To elucidate its molecular features, expression patterns, and subcellular localization.
To establish a foundation for understanding its role in rice.

Main Methods:

Integrated bioinformatics analysis
Spatiotemporal expression profiling
Subcellular localization experiments in *Nicotiana benthamiana*

Main Results:

OsPP2C61 encodes a hydrophilic, basic protein with a canonical PP2C fold.
Phylogenetic analysis places it in the PP2C.D clade with conserved motifs.
It is highly expressed in roots and localizes to the nucleus and plasma membrane.
Promoter analysis reveals ABA, MeJA, and stress-responsive elements.

Conclusions:

This study provides the first comprehensive characterization of OsPP2C61.
OsPP2C61 is a potential candidate gene for enhancing rice improvement.
Further mechanistic studies on OsPP2C61 are warranted.