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Signal Amplification by Reversible Exchange (SABRE) hyperpolarization now enables robust 13C-pyruvate polarization for preclinical metabolic imaging. This advance overcomes previous limitations, making SABRE a practical tool for magnetic resonance applications.

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Area of Science:

  • Magnetic Resonance Imaging (MRI)
  • Hyperpolarization Techniques
  • Metabolic Imaging

Background:

  • Magnetic resonance methods like NMR and MRI face sensitivity limitations.
  • Signal Amplification by Reversible Exchange (SABRE) is a rapid, cost-effective hyperpolarization technique.
  • Previous SABRE methods struggled to polarize biologically relevant molecules like pyruvate due to catalyst binding issues.

Purpose of the Study:

  • To review the advancements in SABRE hyperpolarization for 13C-labeled pyruvate.
  • To synthesize mechanistic insights, catalyst innovations, and field-cycling strategies.
  • To highlight progress towards in vivo applications in metabolic imaging.

Main Methods:

  • Exploration of SABRE mechanistic foundations and limitations of early approaches.
  • Development of DMSO-assisted catalyst activation and subsequent SABRE variants (SABRE-SHEATH, pulsed-field, SLIC-based, LIGHT-SABRE).
  • Investigation of catalyst structure-function, substrate exchange kinetics, and magnetic field effects.

Main Results:

  • Robust polarization of 13C-pyruvate achieved through catalyst innovations and field-cycling strategies.
  • Development of biocompatible formulations, catalyst recycling, and purification workflows.
  • Successful first in vivo metabolic imaging studies using SABRE-polarized pyruvate.

Conclusions:

  • SABRE has evolved from a chemical innovation to a practical hyperpolarization method for pyruvate.
  • SABRE shows potential to complement or surpass dissolution DNP in preclinical metabolic imaging.
  • Continued advancements position SABRE as a key technique for sensitive metabolic studies.