Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Author Correction: Electrified interfacial oxygen-down water boosts efficient and durable electrolysis.

Nature communications·2026

Same author

Electrified interfacial oxygen-down water boosts efficient and durable electrolysis.

Nature communications·2026

Same author

Biomass-derived hard carbon anodes with enhanced capacity for sodium-ion batteries.

Dalton transactions (Cambridge, England : 2003)·2025

Same author

Tracing antibiotics in food through dual-mode ratiometric luminescence core-shell nanoparticles.

Food chemistry·2025

Same author

Mixed Ionic-Covalent Bonds Achieve Durable Acidic Water Oxidation.

Journal of the American Chemical Society·2025

Same author

Patterning and Dynamics of Structured Surfaces in Metal/Covalent Organic Frameworks and Polymer Chemistry.

Topics in current chemistry (Cham)·2025

Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026

Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026

Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026

Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026

Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026

Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

A Dual-Functional Two-Photon PLIM Probe Based on Ruthenium(II) Complex: Sequence Selectivity and Aβ Aggregation

Xufeng Mai¹, Jian Chen¹, Kewei Yong¹

¹School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P. R. China.

Analytical Chemistry

|May 5, 2026

Summary

This summary is machine-generated.

Researchers developed Ru-dmpip, a novel probe for Alzheimer's disease (AD) research. This tool specifically detects β-amyloid (Aβ) aggregation, offering new insights into AD pathology and potential therapeutic strategies.

More Related Videos

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 6, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Biomedical Engineering
Neuroscience
Chemical Biology

Background:

Alzheimer's disease (AD) is characterized by β-amyloid (Aβ) plaque formation.
Existing Aβ probes lack sequence selectivity and real-time aggregation monitoring.
N/C-truncated Aβ fragments are implicated in AD etiology, but their toxic species and mechanisms remain unclear.

Purpose of the Study:

To develop a novel probe for selective detection and real-time monitoring of Aβ aggregation.
To investigate the binding mechanisms and aggregation states of Aβ peptides.
To provide a tool for advancing Alzheimer's disease pathological research.

Main Methods:

Synthesis of Ru-dmpip, a two-photon phosphorescence lifetime imaging microscopy (PLIM) probe.
Evaluation of Ru-dmpip's specificity for Aβ peptides and binding affinity.
In vitro cellular imaging and in vivo imaging in APP-PS1 transgenic mouse brain sections.

Main Results:

Ru-dmpip exhibits high specificity for Aβ_X-42 peptides (Aβ_1-42/Aβ_11-42) over Aβ_1-40.
The probe binds all aggregated states of Aβ_X-42, showing sensitive phosphorescence changes.
Ru-dmpip visualizes Aβ_1-42 aggregation at the single-fiber level and detects oligomers surrounding plaques.

Conclusions:

Ru-dmpip is the first two-photon PLIM probe with sequence selectivity and aggregation monitoring capabilities for Aβ.
The probe facilitates visualization of Aβ aggregation, including previously undetectable oligomeric species.
Ru-dmpip offers a valuable tool for Alzheimer's disease research and inspires the design of new Aβ sensors.