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Related Experiment Video

Updated: May 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Real-time transcriptomic profiling in distinct experimental conditions.

Tamer Butto¹, Stefan Pastore^1,2, Max Müller¹

¹Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg-University Mainz, Mainz, Germany.

|May 5, 2026

Summary

This summary is machine-generated.

Real-time nanopore sequencing enables rapid RNA-seq quality control and differential gene expression analysis. This cost-effective approach identifies expression changes as early as one hour post-sequencing initiation.

Keywords:

RNA quality control S. cerevisiae chromosomes computational biology gene expression human nanopore-seq real-time transcriptomics systems biology

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Last Updated: May 6, 2026

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Published on: March 7, 2018

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Nanopore technology provides real-time sequencing capabilities.
Efficient data management and cost-effectiveness are key advantages.
Real-time analysis offers new possibilities for rapid experimental feedback.

Purpose of the Study:

To demonstrate the versatility of real-time transcriptomics analysis for long-read RNA-seq quality control.
To showcase applications in diverse experimental settings.
To highlight the potential for early detection of gene expression changes.

Main Methods:

Utilized nanopore sequencing for real-time RNA-seq.
Applied real-time analysis for quality control across four experimental setups.
Performed differential gene expression analysis at various time points post-sequencing initiation.

Main Results:

Demonstrated rapid quality control for transcriptome profiling, transcript enrichment, heat shock response, and gene manipulation studies.
Identified sample/condition variability and gene counts in real-time.
Detected differentially expressed genes/transcripts as early as 1 hour post-sequencing initiation, with consistent observations throughout the run.

Conclusions:

Real-time nanopore transcriptomics analysis is a versatile tool for rapid quality checks.
This approach enables cost-effective and efficient experimental validation.
Potential integration into clinical applications is foreseeable.