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A near-infrared AIEgens-based fluorescent probe with large stokes shift for selective lysine detection: Photophysical
Xin Wang1, Wenhui Xue1, Penghui Xia1
1School of Chemistry and Materials Engineering, Anhui Provincial Key Laboratory of Innovative Drug Development and Industrial Integration Jointly Established Discipline, Anhui Provincial Key Laboratory of Green Carbon Chemistry, Engineering Research Center of Biomass Conversion and Pollution Prevention of Anhui Educational Institutions, Fuyang Normal University, Fuyang, Anhui Province 236037, PR China.
Abstract:
Lysine is an essential amino acid and must be obtained from external sources. Therefore, establishing the method for Lys detection is of great significance. As the near-infrared fluorescent probe, DBH with aggregation-induced emission characteristics was designed, synthesized and characterized, which it's worth noting that the recognition process features a large Stokes shift of 225 nm. DBH exhibits high selectivity, anti-interference capability, and stability toward lysine. And its LOD toward Lys was low to 0.475 μM. The mechanism between them was investigated through some method to confirm that DBH and lysine interacted with 1:3 stoichiometric ratio. And it's interesting that DBH also achieves specific chiral transduction for D/l-lysine. Furthermore, a portable smartphone was employed for qualitative and quantitative detection of lysine in food samples. DBH has visualized lysine in vivo, indicating its potential value in bioimaging research. Future studies could explore the application of DBH in the analysis of biological fluids.
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