Real-time Dual Sensing of cTnI and Myoglobin via Printable Graphitized Electrodes

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Cardiovascular Research

Background:

• Acute myocardial infarction (AMI) is a major global health issue with high mortality rates.
• Early and pre-symptomatic detection of AMI is critical for effective clinical intervention.
• Simultaneous monitoring of key biomarkers like cardiac troponin I (cTnI) and myoglobin is essential for timely AMI diagnosis.

Purpose of the Study:

• To develop an ultra-sensitive and highly specific electrochemical biosensor for dual detection of cTnI and myoglobin.
• To address the need for simultaneous biomarker monitoring for improved AMI diagnosis.
• To create a practical and reliable platform for real-time AMI biomarker detection.

Main Methods:

• Fabrication of a laser-induced graphene (LIG)-based electrochemical biosensor.
• Utilized electrochemical analytical techniques, including Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV).
• Validated sensor performance using physicochemical characterizations and testing in human blood serum.

Main Results:

• Achieved ultra-low limits of detection (LoD) for cTnI (0.035 ng/mL) and myoglobin (1.39 ng/mL).
• Demonstrated excellent stability and reproducibility of the fabricated electrodes.
• Successfully validated the dual-detection platform in human blood serum with high recovery rates.

Conclusions:

• The developed LIG-based electrochemical biosensor provides a promising platform for simultaneous dual detection of cTnI and myoglobin.
• This technology holds significant potential for practical, real-world applications in early and accurate AMI diagnosis.
• The sensor's high sensitivity, specificity, and performance in serum pave the way for improved cardiovascular patient care.