Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolomics-based elucidation of the chemical basis under-lying seasonal variations in sensory quality of Goldsands Black Tea.

Frontiers in plant science·2026
Same author

Surgical Outcomes of Perioperative Toripalimab in Stage III Resectable Non-Small Cell Lung Cancer: Post Hoc Analysis of the Neotorch Randomized Clinical Trial.

JAMA surgery·2026
Same author

The patient journey of adolescents and young adults with thyroid cancer: A social media listening study in China.

Asia-Pacific journal of oncology nursing·2026
Same author

[An electroencephalogram-based emotion recognition method using multi-branch convolutional neural networks and Transformer].

Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi·2026
Same author

Extended Preoperative Immunotherapy Duration Reduces the Risk of Myasthenia Gravis Exacerbation for Patients with Thymoma: A Multicenter Retrospective Cohort Study.

Neurology and therapy·2026
Same author

Online Health Information Seeking Behaviors Among People With Diabetes: A Scoping Review.

SAGE open nursing·2026

Related Experiment Video

Updated: May 8, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

GhWRKY70-GhXDH1 module enhances drought tolerance in cotton by maintaining ROS homeostasis.

Yuyang Xiao1, Menghan Liu2, Lulu Chen1

  • 1Plant Genomics and Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.

Plant Physiology and Biochemistry : PPB
|May 6, 2026
PubMed
Summary

This study reveals how the GhWRKY70 gene enhances drought tolerance in cotton. Overexpression boosts tolerance, while its absence reduces it, highlighting a key regulatory role in plant stress response.

Keywords:
CottonDroughtGhWRKY70GhXDH1ROS

More Related Videos

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions
15:30

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

Published on: August 5, 2020

Related Experiment Videos

Last Updated: May 8, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
10:18

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Published on: August 20, 2011

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions
15:30

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

Published on: August 5, 2020

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Agricultural Science

Background:

  • WRKY transcription factors are crucial for plant stress responses.
  • Direct validation of cotton WRKY genes under drought using stable transformants is limited.

Purpose of the Study:

  • To functionally characterize the drought-responsive cotton gene GhWRKY70.
  • To elucidate the GhWRKY70-mediated regulatory mechanism for drought tolerance in cotton.

Main Methods:

  • Gene expression analysis (RNA-sequencing) in GhWRKY70 overexpression lines and wild type cotton.
  • Physiological assays measuring antioxidant enzyme activities and total antioxidant capacity.
  • Gene silencing using Virus-Induced Gene Silencing (VIGS) to validate downstream targets.

Main Results:

  • GhWRKY70 overexpression enhanced drought tolerance in cotton, while ghwrky70 mutants showed reduced tolerance.
  • RNA-seq identified oxidative stress-related pathways regulated by GhWRKY70.
  • GhWRKY70 directly targets GhXDH1, a xanthine dehydrogenase-like gene, which positively contributes to drought tolerance.

Conclusions:

  • A novel GhWRKY70-GhXDH1 regulatory module enhances drought resistance in cotton.
  • This finding provides a mechanistic basis for developing drought-tolerant cotton cultivars.