Molecular Methods: Analytical Principles and Test Design Considerations
Mine M Yilmaz1, Sidra Zaheer2, Mahesh M Mansukhani3
1Department of Pathology, Icahn School of Medicine at Mount Sinai, 1468 Madison Ave., Annenberg Building 15th Fl, New York NY 10029, USA.
Abstract:
In this article, the authors review the types of molecular alterations present in cancer and molecular methods used commonly in clinical diagnostic laboratories, including technologies such as next-generation sequencing and digital polymerase chain reaction. Additionally, the authors discuss considerations for test design, incorporating factors such as specimen type and biomarker type, to design a molecular test menu that delivers accurate and timely results in an efficient and cost-effective manner.
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