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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 8, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Super-resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa).

Sevannah A Steeves1, Saber H Saber1,2, Mo Chen1,3

  • 1Clem Jones Centre for Ageing Dementia Research (CJCADR), Queensland Brain Institute, The University of Queensland, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2026
PubMed
Summary
This summary is machine-generated.

Super-resolution spinning-disk confocal microscopy with optical photon reassignment (SoRa) achieves enhanced resolution for live-cell imaging. This fast, low-phototoxicity technique aids drug discovery and biological research.

Keywords:
Confocal microscopyLive imagingMitochondrial traffickingNeuronsSuper-resolution

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Published on: August 4, 2018

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Conventional fluorescence microscopy is limited by diffraction.
  • Super-resolution techniques offer enhanced resolution but often require complex sample preparation or processing.
  • Live-cell imaging demands speed and low phototoxicity.

Purpose of the Study:

  • To introduce and highlight the capabilities of Super-resolution spinning-disk confocal microscopy with optical photon reassignment (SoRa).
  • To demonstrate SoRa's utility in high-throughput screening and high-resolution 3D live-cell imaging.
  • To showcase SoRa as a versatile tool for biological and drug discovery research.

Main Methods:

  • Utilized Super-resolution spinning-disk confocal microscopy with optical photon reassignment (SoRa).
  • Applied SoRa for high-throughput screening of drug effects on neuronal processes.
  • Employed SoRa for high-resolution 3D live-cell imaging, including mitochondrial trafficking in neurons.

Main Results:

  • SoRa extends fluorescence microscopy resolution beyond the diffraction limit.
  • Achieved enhanced lateral and axial resolution while maintaining rapid imaging speeds.
  • Demonstrated low phototoxicity enabling real-time capture of dynamic cellular events.

Conclusions:

  • SoRa is a powerful super-resolution technique suitable for live-cell imaging and high-throughput studies.
  • Its speed, 3D imaging capability, and low phototoxicity make it ideal for dynamic cellular processes.
  • SoRa's compatibility with standard dyes enhances its applicability in diverse research areas.