Related Experiment Videos
Salidroside and Hongjingtian Injection Inhibit the Onset and Progression of Asthma via Pyroptosis in the
Zhidan Lu1,2,3,4, Jingwen Li1,2,3, Shujie Hou1,2,3
1The First Department of Pulmonary and Critical Care Medicine, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, Hebei, China, hebmu.edu.cn.
Background:
Ozone is a common air pollutant, and exposure to high concentrations of ozone can promote the onset and progression of asthma. Pyroptosis is a form of cell death associated with asthma exacerbation.
Methods:
This study aimed to identify ozone-related pyroptosis genes through network toxicology and RNA-seq analysis and to investigate the role of pyroptosis genes in asthma using multiple asthma tissue samples and machine learning methods. Key pyroptosis genes with significant roles in asthma were identified through experiments and multiomics approaches. Using network pharmacology methods, we screened traditional Chinese medicine (TCM) components associated with key pyroptosis genes. Through molecular clustering and molecular docking, we explored the interaction between TCM components and key genes. Using animal model drug interventions, we further analyzed the therapeutic effects of TCM components targeting pyroptosis on asthma under ozone exposure.
Results:
This study identified 16 ozone-related pyroptosis genes through network pharmacology and transcriptomics data analysis. Based on multiple asthma tissue samples and machine learning methods, the 16 pyroptosis genes were found to have diagnostic predictive roles in asthma. Through experimentation and multiomics approaches, gasdermin D (GSDMD) was identified as a key gene in exacerbating asthma under ozone exposure. Furthermore, scRNA-seq and virtual knockout revealed that GSDMD exerts a pro-inflammatory effect on the asthma microenvironment. Using network pharmacology methods, we screened for salidroside, a Chinese herbal component related to GSDMD. Through molecular clustering and molecular docking, we identified seven salidroside analogs in Hongjingtian injection (HJT). Using an animal model drug intervention, we further analyzed the intervention effects of salidroside and HJT on asthma under ozone exposure.
Conclusions:
Ozone promotes the onset and progression of asthma by exacerbating inflammation and pyroptosis. salidroside and its analogs can target GSDMD to inhibit asthma exacerbation in ozone-induced inflammation environments.
Related Concept Videos
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
01:19Antiasthma Drugs: Leukotriene Modifiers
Leukotriene modifiers work through two distinct mechanisms:
01:25Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
01:20Asthma: Pathogenesis and Management
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
01:24Antiasthma Drugs: Methylxanthines
Theophylline is thought to inhibit phosphodiesterase enzymes, increasing intracellular levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). This rise in cAMP and cGMP concentrations stimulates cardiac function,...
01:25Antiasthma Drugs: β2-Adrenoceptor Agonists
One class of bronchodilators includes β2-adrenoceptor agonists. These agents target the β2-adrenoceptors located on bronchial smooth muscle cells. By stimulating these receptors, β2-agonists induce relaxation in these...