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Related Experiment Video

Updated: May 8, 2026

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
11:44

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Published on: March 24, 2015

Unified platform for multiplex immunofluorescence across liver tissues and engineered models.

Bianca Franco Leonardi1,2, Guo Yin1, Natalja Amiridze1

  • 1Department of Hepatology and Gastroenterology, Campus Virchow-Klinikum and Campus Charité Mitte, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Egastroenterology
|May 7, 2026
PubMed
Summary

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eGastroenterology·2026

Researchers developed a new multiplex immunofluorescence workflow for analyzing liver tissues and cells. This method simplifies complex techniques, making advanced cellular analysis accessible for studying liver diseases.

Area of Science:

  • Hepatology
  • Immunohistochemistry
  • Digital Pathology

Background:

  • Multiplex immunostaining and digital image analysis are crucial for studying liver disease microenvironments.
  • Technological complexity, specialized equipment, and incompatibility with standard workflows hinder widespread adoption of multiplexing platforms.
  • Existing methods often do not work with archival formalin-fixed paraffin-embedded (FFPE) tissues or in vitro cell cultures.

Purpose of the Study:

  • To develop a user-friendly multiplex immunofluorescence workflow adaptable to various sample types.
  • To enable detailed spatial mapping of cellular markers in liver research.
  • To overcome barriers preventing the integration of multiplexing into routine laboratory practices.

Main Methods:

  • A customized immunofluorescence workflow using sequential antibody stripping was created for FFPE liver sections, organoids, and primary mouse liver cells.
Keywords:
Image Processing, Computer-AssistedLiver DiseasesMicrophysiological SystemsMicroscopy, FluorescenceOrganoids

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High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

Related Experiment Videos

Last Updated: May 8, 2026

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
11:44

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Published on: March 24, 2015

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

  • Cells were cultured in 2D systems or a biliary niche-on-a-chip platform.
  • An open-source, Python-based software tool was used for image processing and channel alignment.

    • Main Results:

    • The protocol successfully generated multiplexed digital images from diverse FFPE liver samples and cell cultures.
    • The workflow visualizes antigen-expressing cells and protein-protein interactions via proximity ligation assays.
    • Technical considerations for integrating this method into standard immunohistochemistry workflows are detailed.

    Conclusions:

    • This methodology integrates multiplex immunofluorescence into standard laboratory workflows.
    • It enables researchers to overcome challenges related to technological complexity and cost.
    • Facilitates the transition from conventional to multiplexed microscopy for broader accessibility.