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Updated: May 9, 2026
10:20
In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
Intestinal Source Control of Lipid Metabolism by Enzyme-Probiotic Encapsulated, Spatiotemporal Crosslinked, and Small
Xiaolin Wu1, Weiwen Liang2, Siqi He3
1Department of General Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, P. R. China.
Summary
A novel enzyme-probiotic biohybrid system, AKK-COD, encapsulated in hydrogel microspheres, effectively delivers probiotics to the small intestine. This approach improves lipid metabolism and reduces high-fat diet-induced hypercholesterolemia.
Area of Science:
- Biotechnology
- Microbiology
- Gastroenterology
Background:
- Probiotic therapy for lipid metabolism regulation faces challenges in targeted delivery and colonization.
- Effective spatiotemporal delivery is crucial for probiotic efficacy in the small intestine.
Purpose of the Study:
- To develop an enzyme-probiotic biohybrid (AKK-COD) encapsulated in hydrogel microspheres for enhanced lipid metabolism regulation.
- To overcome challenges in probiotic delivery and colonization efficiency in the small intestine.
Main Methods:
- Development of an enzyme-probiotic biohybrid (AKK-COD) encapsulated in thiol-modified alginate hydrogel microspheres.
- Evaluation of intragastric protection, small intestinal adhesion, and controlled release of AKK-COD.
- In vivo assessment of the system's efficacy in alleviating high-fat diet-induced hypercholesterolemia.
Main Results:
- The hydrogel microspheres provided intragastric protection and adhered to the small intestinal mucosa.
- Controlled release of AKK-COD modulated lipid absorption pathways and promoted microbial homeostasis.
- Significant alleviation of high-fat diet-induced hypercholesterolemia was observed in vivo.
Conclusions:
- The developed enzyme-probiotic biohybrid system offers a safe and efficient strategy for lipid-lowering therapies.
- This approach demonstrates potential for targeted probiotic delivery and improved lipid metabolism regulation.
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