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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

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Related Experiment Video

Updated: May 10, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
08:26

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

Long non-coding RNA RsLNC2361 positively regulate anthocyanin accumulation in radish.

Haiyi Liu1,2, Xiaobo Luo3, Linjun Wu1,2

  • 1College of Agriculture, Guizhou University, Guiyang, Guizhou, 550025, China.

BMC Plant Biology
|May 8, 2026
PubMed
Summary

Long non-coding RNA (lncRNA) RsLNC2361 positively regulates anthocyanin accumulation in radish. This discovery offers insights into breeding radishes with enhanced anthocyanin content for improved nutritional value.

Keywords:
RsLNC2361AnthocyaninsMetabolomeRadishTranscriptome

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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

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Related Experiment Videos

Last Updated: May 10, 2026

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Published on: June 25, 2018

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Area of Science:

  • Plant molecular biology
  • Genomics
  • Biochemistry

Background:

  • Radish (Raphanus sativus L.) is a vital Brassicaceae vegetable crop with significant nutritional and medicinal benefits.
  • Anthocyanin-rich radish varieties are highly sought after for their vibrant color and health-promoting properties.
  • The regulatory role of long non-coding RNA (lncRNA) in anthocyanin accumulation in radish remains underexplored.

Purpose of the Study:

  • To investigate the function of the differentially expressed lncRNA RsLNC2361 in regulating anthocyanin biosynthesis in radish.
  • To elucidate the molecular mechanism underlying RsLNC2361-mediated anthocyanin accumulation.

Main Methods:

  • LncRNA sequencing to identify differentially expressed lncRNAs.
  • Gene overexpression and virus-induced gene silencing (VIGS) to assess RsLNC2361 function.
  • Transcriptome and metabolome sequencing to analyze gene expression and metabolite profiles.
  • Bioinformatic analysis to predict lncRNA-miRNA interactions.

Main Results:

  • RsLNC2361 was found to be differentially expressed between red and white skin radish.
  • Overexpression of RsLNC2361 promoted anthocyanin accumulation, while silencing decreased it, confirming a positive regulatory role.
  • RsLNC2361 modulated the expression of anthocyanin structural genes.
  • Integrated transcriptomic and metabolomic analyses revealed enrichment of pathways related to flavonoid and phenylpropanoid biosynthesis.
  • miRNA858 was predicted to target RsLNC2361, suggesting a potential competitive endogenous RNA (ceRNA) mechanism.

Conclusions:

  • RsLNC2361 plays a crucial role in promoting anthocyanin accumulation in radish.
  • This study reveals a novel regulatory pathway involving lncRNA in anthocyanin biosynthesis.
  • The findings provide a theoretical foundation for breeding radish varieties with enhanced anthocyanin content.