Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolated hydrometrocolpos and cloacal malformation: can we prenatally distinguish them?-A case report and literature review.

Archives of gynecology and obstetrics·2025
Same author

Creatine maintains intestinal homeostasis and protects against colitis.

Proceedings of the National Academy of Sciences of the United States of America·2017
Same author

Asymmetries of Dark and Bright Negative Afterimages Are Paralleled by Subcortical ON and OFF Poststimulus Responses.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2017
Same author

A Method for Removal of CO from Exhaust Gas Using Pulsed Corona Discharge.

Journal of the Air & Waste Management Association (1995)·2017
Same author

Fluorescent Strips of Electrospun Fibers for Ratiometric Sensing of Serum Heparin and Urine Trypsin.

ACS applied materials & interfaces·2017
Same author

Predicting potential drug-drug interactions by integrating chemical, biological, phenotypic and network data.

BMC bioinformatics·2017

Related Experiment Video

Updated: May 10, 2026

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
07:55

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Published on: December 13, 2019

0.1% Peracetic Acid Treatment Is the Optimal Process for Ovarian Decellularized Extracellular Matrix.

MeyLign Long1, Anita Touch1, You Yang1

  • 1Reproductive Medical Department of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, Sichuan University, Chengdu, China.

Tissue Engineering. Part C, Methods
|May 9, 2026
PubMed
Summary

0.1% peracetic acid (PAA) is the optimal sterilization method for ovarian decellularized extracellular matrix (OV-dECM). It demonstrated superior sterilization and biocompatibility, promoting tissue integration and favorable macrophage responses in vivo.

Keywords:
biocompatibilityovarian decellularized extracellular matrixsterilization

More Related Videos

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
08:10

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

Published on: October 7, 2013

Related Experiment Videos

Last Updated: May 10, 2026

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs
07:55

Pancreatic Tissue-Derived Extracellular Matrix Bioink for Printing 3D Cell-Laden Pancreatic Tissue Constructs

Published on: December 13, 2019

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix
08:10

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

Published on: October 7, 2013

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Sterilization Techniques

Background:

  • Decellularized extracellular matrix (dECM) is a promising biomaterial for tissue regeneration.
  • Sterilization is crucial for dECM safety and efficacy, but it can affect material properties.
  • Ovarian dECM (OV-dECM) requires optimized sterilization for potential therapeutic applications.

Purpose of the Study:

  • To determine the optimal sterilization process for OV-dECM.
  • To evaluate the sterilization efficiency and biocompatibility of different methods.
  • To assess the impact of sterilization on OV-dECM's suitability for regenerative medicine.

Main Methods:

  • OV-dECM preparation followed by four sterilization methods: UV, CuCl2/H2O2, 70% ethanol, and 0.1% peracetic acid (PAA).
  • Sterilization efficiency assessed via microbial testing.
  • In vitro biocompatibility evaluated using human umbilical cord mesenchymal stem cells (viability, morphology, proliferation).
  • In vivo biocompatibility assessed in mice, analyzing neovascularization (CD31, α-SMA) and macrophage polarization (CD68, CD86, CD206).

Main Results:

  • 0.1% PAA and 70% ethanol showed effective sterilization.
  • Both 0.1% PAA and 70% ethanol groups supported stem cell integration, viability, and morphology.
  • Neovascularization markers (CD31, α-SMA) indicated good vascularization potential, with higher α-SMA in the 0.1% PAA group.
  • 0.1% PAA treatment promoted M1-M2 macrophage polarization more effectively than 70% ethanol in vivo.

Conclusions:

  • 0.1% peracetic acid (PAA) is the optimal sterilization method for OV-dECM.
  • PAA sterilization ensures high sterilization efficiency and excellent biocompatibility.
  • This optimized OV-dECM supports cell integration, vascularization, and favorable immune responses, making it suitable for regenerative applications.