Related Experiment Video
Updated: May 12, 2026
High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato
Published on: June 7, 2024
Insights into the regulatory role of pulsed electric field-induced ROS burst in metabolite changes during potato
Renjie Zhao1, Qiannan Liu1, Jing Li1
1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Integrated Laboratory of Potato Staple Food Processing Technology, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Product Processing and Storage, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China.
Abstract:
Traditional electroporation theory cannot fully explain metabolite accumulation induced by pulsed electric field (PEF) treatment during fruit and vegetable processing. This study investigated reactive oxygen species (ROS)-mediated metabolite changes in potatoes under PEF treatment. A rapid, electric field strength-dependent ROS burst was observed, with O₂•- and H₂O₂ increased by 194% and 104%, respectively, at 1.6 kV/cm. Antioxidant enzyme-related genes were upregulated, and the mitogen-activated protein kinase (MAPK) signaling pathway was activated, confirming oxidative stress. The numbers of differentially expressed genes (DEGs) and metabolites (DEMs) identified by transcriptomic and metabolomic analyses increased with electric field strength. iPath analysis showed that DEGs and DEMs were mainly enriched in amino acid, carbohydrate, lipid, and polyphenol metabolism, and related gene-metabolite networks were constructed. These metabolic changes suggest that PEF may modulate flavor-related composition and enhance antioxidant potential in potatoes products. This study provides novel insights into ROS-mediated metabolic reprogramming induced by PEF.
