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Published on: September 29, 2023
Omics-Based Insights into Polysaccharide Biosynthesis in Macrofungi
Xing Luo1,2, Ji-Hang Jiang1, Li-Wei Zhou3
1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
This study introduces a multi-omics method to understand polysaccharide biosynthesis in medicinal fungi. The protocol helps identify key genes for pathway elucidation and strain improvement.
Area of Science:
- Mycology
- Biochemistry
- Genomics
Background:
- Medicinal macrofungi are vital sources of bioactive compounds.
- Polysaccharide biosynthesis pathways in these fungi remain poorly understood.
- This knowledge gap limits the potential for fungal biotechnology and drug discovery.
Purpose of the Study:
- To develop and present an integrated multi-omics protocol.
- To elucidate polysaccharide biosynthetic pathways in medicinal macrofungi.
- To provide a framework for guiding fungal strain improvement.
Main Methods:
- Experimental design and de novo genome assembly.
- Transcriptomic and metabolomic profiling.
- Weighted gene co-expression network analysis, KEGG pathway mapping, and RT-qPCR validation.
Main Results:
- Successfully established a comprehensive multi-omics workflow.
- Identified key genes involved in polysaccharide biosynthesis.
- Validated the pipeline's effectiveness for pathway elucidation.
Conclusions:
- The presented protocol offers a robust framework for studying fungal secondary metabolism.
- This approach facilitates the identification of novel biosynthetic genes.
- Enables targeted strain improvement for enhanced polysaccharide production.
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