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Related Concept Videos

Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Fungal Group Zygomycota01:29

Fungal Group Zygomycota

Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...

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Related Experiment Video

Updated: May 12, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
07:12

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Omics-Based Insights into Polysaccharide Biosynthesis in Macrofungi.

Xing Luo1,2, Ji-Hang Jiang1, Li-Wei Zhou3

  • 1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2026
PubMed
Summary

This study introduces a multi-omics method to understand polysaccharide biosynthesis in medicinal fungi. The protocol helps identify key genes for pathway elucidation and strain improvement.

Keywords:
Bioactive compoundsMedicinal macrofungiMetabolic networkStrategic bioresource

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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

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Last Updated: May 12, 2026

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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
09:37

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Area of Science:

  • Mycology
  • Biochemistry
  • Genomics

Background:

  • Medicinal macrofungi are vital sources of bioactive compounds.
  • Polysaccharide biosynthesis pathways in these fungi remain poorly understood.
  • This knowledge gap limits the potential for fungal biotechnology and drug discovery.

Purpose of the Study:

  • To develop and present an integrated multi-omics protocol.
  • To elucidate polysaccharide biosynthetic pathways in medicinal macrofungi.
  • To provide a framework for guiding fungal strain improvement.

Main Methods:

  • Experimental design and de novo genome assembly.
  • Transcriptomic and metabolomic profiling.
  • Weighted gene co-expression network analysis, KEGG pathway mapping, and RT-qPCR validation.

Main Results:

  • Successfully established a comprehensive multi-omics workflow.
  • Identified key genes involved in polysaccharide biosynthesis.
  • Validated the pipeline's effectiveness for pathway elucidation.

Conclusions:

  • The presented protocol offers a robust framework for studying fungal secondary metabolism.
  • This approach facilitates the identification of novel biosynthetic genes.
  • Enables targeted strain improvement for enhanced polysaccharide production.