Related Experiment Video
Updated: May 12, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
Published on: September 29, 2023
Omics-Based Insights into Polysaccharide Biosynthesis in Macrofungi
Xing Luo1,2, Ji-Hang Jiang1, Li-Wei Zhou3
1State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Abstract:
To address the limited understanding of polysaccharide biosynthesis in medicinal macrofungi, we present an integrated multi-omics protocol. Our method details the entire workflow, from experimental design and de novo genome assembly to transcriptomic and metabolomic profiling. Key genes are pinpointed through weighted gene co-expression network analysis and KEGG pathway mapping, followed by experimental validation using RT-qPCR. This established pipeline provides a robust framework for elucidating biosynthetic pathways and guiding strain improvement.
More Related Videos
12:02Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
09:37Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
Related Concept Videos
Biosynthesis of Polysaccharides
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Formation of Lipopolysaccharides
Fungal Group Zygomycota