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Advanced Evaluation of T-Cell-Dependent Bispecific Antibodies Using Fluorescence Imaging and In Vivo Models
Mikiko Itsukaichi1, Masahiro Yasunaga2
1Division of Developmental Therapeutics, EPOC, National Cancer Center, Kashiwa, Chiba, Japan.
In this chapter, advanced methods for evaluating T-cell-dependent bispecific antibodies (TDBs) using in vivo fluorescence imaging and animal models are presented. TDBs, which target tumor-associated antigens on cancer cells and the TCR/CD3 complex on T cells, have shown success in hematologic malignancies but face challenges in solid tumors due to factors like reduced T-cell quality and low tumor-infiltrating T cells. Comprehensive protocols for cancer and T-cell preparation, flow cytometry, xenograft model generation, and imaging techniques are outlined, aiming to enhance the understanding of TDB dynamics and improve their efficacy in solid tumor treatment.
In this chapter, advanced methods for evaluating T-cell-dependent bispecific antibodies (TDBs) using in vivo fluorescence imaging and animal models are presented. TDBs, which target tumor-associated antigens on cancer cells and the TCR/CD3 complex on T cells, have shown success in hematologic malignancies but face challenges in solid tumors due to factors like reduced T-cell quality and low tumor-infiltrating T cells. Comprehensive protocols for cancer and T-cell preparation, flow cytometry, xenograft model generation, and imaging techniques are outlined, aiming to enhance the understanding of TDB dynamics and improve their efficacy in solid tumor treatment.
