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Related Concept Videos

Antifungal Agents01:15

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...

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Isolation and Purification of Fungal &#946;-Glucan as an Immunotherapy Strategy for Glioblastoma
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Six Undescribed Lanostane-Type Triterpenoids From Ganoderma lucidum.

Ze Liu1, Ping Ying2, Wei-Ming Huang1

  • 1Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.

Chemistry & Biodiversity
|May 11, 2026
PubMed
Summary

Researchers isolated six new lanostane triterpenoids and 21 known analogues from Ganoderma lucidum. These compounds, including lucidenate and ganoderate types, showed no significant anti-inflammatory activity in preliminary tests.

Keywords:
Ganoderma lucidumlanostane triterpenoidsstructural elucidation

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Area of Science:

  • Natural Product Chemistry
  • Mycology
  • Medicinal Chemistry

Background:

  • Ganoderma lucidum, a medicinal fungus, is a rich source of bioactive compounds.
  • Lanostane triterpenoids are a class of natural products with diverse biological activities.
  • Understanding the chemical diversity of G. lucidum is crucial for discovering new therapeutic agents.

Purpose of the Study:

  • To isolate and characterize novel lanostane triterpenoids from Ganoderma lucidum.
  • To determine the structures and absolute configurations of the new compounds.
  • To evaluate the anti-inflammatory potential of the isolated compounds.

Main Methods:

  • Isolation of compounds using chromatographic techniques.
  • Structure elucidation via High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS) and 1D & 2D Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Determination of absolute configurations using Electronic Circular Dichroism (ECD) calculations, modified Mosher's method, and X-ray crystallography.

Main Results:

  • Six new lanostane triterpenoids (compounds 1-6) were identified, including lucidenate-type (1-3) and ganoderate-type (4-6) derivatives.
  • Twenty-one known analogues (7-27) were also isolated.
  • None of the isolated compounds exhibited significant inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages, indicating a lack of notable anti-inflammatory activity in this assay.

Conclusions:

  • The study successfully identified and characterized novel lanostane triterpenoids from Ganoderma lucidum.
  • The structural elucidation employed advanced spectroscopic and crystallographic methods.
  • The evaluated compounds did not demonstrate significant anti-inflammatory effects in the tested in vitro model.