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Lactate Monitoring using Fluorescence with Stable Boronic Acid-Functionalized Nanoparticles from

Morvarid H Balouchi1, Zixiao Liu2, Fumi Ishizuka2

  • 1Health, Education and Society, Knowledge Exchange and Research Institute (HES KERI)and School of Life Sciences, Pharmacy and Chemistry, Kingston University, Penrhyn Road, Kingston upon ThamesKT1 2EE, United Kingdom.

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Summary
This summary is machine-generated.

A novel fluorescence indicator displacement assay (FIDA) using boronic acid nanoparticles enables higher detection of L-lactate, crucial for disease monitoring and athletic performance. This synthetic sensor surpasses commercial limitations.

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Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • L-lactate monitoring is vital in clinical diagnostics, the food industry, and sports science.
  • Current enzyme-based lactate monitors have limited detection ranges (<50 mM) and robustness.
  • Boronic acid (BA) based synthetic sensors offer superior performance for lactate detection.

Purpose of the Study:

  • To investigate the fluorescence indicator displacement assay (FIDA) for lactate detection using BA-functionalized nanoparticles.
  • To develop a robust synthetic sensor for high-concentration L-lactate monitoring.
  • To characterize the performance of the developed sensor.

Main Methods:

  • Synthesis of core-shell nanoparticles via RAFT dispersion PISA, functionalized with BA.
  • Utilizing Alizarin Red S (ARS) as a fluorescent indicator and L-lactate as a quencher.
  • Applying the Benesi-Hildebrand approach to determine binding constants and Stern-Volmer analysis for quenching studies.

Main Results:

  • Developed stable BA-functionalized nanoparticles capable of detecting L-lactate via FIDA.
  • Achieved a significantly higher detection range for L-lactate (4.0–625 mM) compared to commercial monitors.
  • Demonstrated that sensor performance (Stern-Volmer constant) is independent of nanoparticle degree of polymerization and is enhanced by ethanol addition.

Conclusions:

  • The developed BA-nanoparticle FIDA system offers a robust and sensitive method for high-concentration L-lactate detection.
  • This synthetic sensor technology holds promise for applications in disease state monitoring and athletic performance management.
  • The FIDA approach provides a viable alternative to conventional enzyme-based lactate assays.