Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radial arterial versus left ventricular dP/dt for assessment of contractility in patients undergoing transcatheter aortic valve replacement: a retrospective observational study.

BMC cardiovascular disorders·2026
Same author

Comparative Evaluation of Functional Aqueous Humor Outflow in Primary Open Angle Glaucoma versus Primary Angle Closure Glaucoma using Aqueous Angiography.

Journal of glaucoma·2026
Same author

Repeat Exposures to Spaceflight or Bed Rest and Spaceflight-Associated Neuro-Ocular Syndrome Findings.

JAMA ophthalmology·2026
Same author

Aqueous Humour Outflow Assessment Using Imaging-Based Techniques in Angle-Based Minimally Invasive Glaucoma Surgery (MIGS): A Systematic Review.

Clinical & experimental ophthalmology·2026
Same author

Structural Characteristics of Parapapillary Choroidal Microvasculature Dropout in Primary Open-Angle Glaucoma: Comparison Between Width and Area.

Clinical ophthalmology (Auckland, N.Z.)·2026
Same author

Machine-Learning-Based Automated Schlemm's Canal Volumetric Segmentation for Optical Coherence Tomography.

Chemical & biomedical imaging·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: May 12, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Vis-OCT Explorer: an open-source software for visible-light optical coherence tomography data processing.

Weijia Fan1, Fengyuanshan Xu1, Roman Kuranov1,2

  • 1Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.

Biomedical Optics Express
|May 11, 2026
PubMed
Summary
This summary is machine-generated.

We created Vis-OCT Explorer, open-source software for high-resolution visible-light OCT imaging. This tool ensures reliable quantitative measurements for retinal imaging, aiding clinical studies.

More Related Videos

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia
06:15

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

Related Experiment Videos

Last Updated: May 12, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia
06:15

Anterior High-Resolution Optical Coherence Tomography in the Diagnosis and Therapeutic Monitoring of Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

Area of Science:

  • Ophthalmology
  • Biomedical Imaging
  • Software Engineering

Background:

  • Visible-light optical coherence tomography (vis-OCT) offers superior retinal imaging resolution.
  • Clinical application of vis-OCT is hindered by the need for advanced post-processing techniques.
  • High-quality, dependable vis-OCT imaging requires specialized reconstruction and analysis methods.

Purpose of the Study:

  • To develop and validate Vis-OCT Explorer, an open-source Python software package for vis-OCT image reconstruction and post-processing.
  • To enhance the quality and reliability of vis-OCT images through advanced processing techniques.
  • To support standardization and multicenter clinical studies using vis-OCT data.

Main Methods:

  • Developed Vis-OCT Explorer, a modular Python package incorporating standard OCT processing steps.
  • Implemented dual-spectrometer balanced detection and STFT-based dispersion compensation optimization.
  • Utilized GPU acceleration for enhanced processing speed and efficiency.
  • Evaluated reconstruction performance using an intensity-based tissue contrast index and assessed measurement repeatability via ICC analysis.

Main Results:

  • Balanced detection significantly improved vis-OCT image quality.
  • Vis-OCT Explorer demonstrated good repeatability for retinal nerve fiber layer (RNFL) and ganglion cell-inner plexiform layer (GCIPL) thickness measurements (ICC > 0.8).
  • High cross-user repeatability was confirmed for both macula- and optic nerve head-centered images.

Conclusions:

  • Vis-OCT Explorer provides high-quality image reconstruction for reliable quantitative measurements in vis-OCT.
  • The software facilitates dependable quantitative analysis, crucial for clinical ophthalmology.
  • This validated tool supports future multicenter vis-OCT studies and data standardization efforts.