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Updated: Sep 30, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Published on: March 24, 2016
Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry
Jeffrey M Spraggins1,2,3,4,5, Amanda Hummon6,7, Shane R Ellis8
1Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
Abstract:
Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful molecular imaging technology that is capable of imaging a wide range of molecular classes at cellular resolution. This Primer includes an overview of basic imaging mass spectrometry concepts, instrumentation, processing approaches, and advanced applications. We also address key challenges and considerations with an eye on how to optimize instrumentation and methods to overcome issues with spatial resolution, sensitivity, and specificity.
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