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Related Concept Videos

Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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Related Experiment Video

Updated: May 7, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
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Extracellular vesicle analysis.

An Hendrix1,2, Lien Lippens1,2,3, Cláudio Pinheiro1,2,3

  • 1Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.

Nature Reviews. Methods Primers
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Summary
This summary is machine-generated.

Extracellular vesicles (EVs) are key for cell communication and hold diagnostic/therapeutic potential. This primer guides EV analysis, method selection, and interpretation across diverse biological sources.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Biotechnology

Background:

  • Cells communicate via extracellular vesicles (EVs), phospholipid-bound particles with roles in health and disease.
  • EVs show promise for diagnostics and therapeutics, driving technological advancements in their study.
  • The variety of EV isolation and characterization methods presents challenges for researchers.

Purpose of the Study:

  • To provide guidance for extracellular vesicle (EV) analysis across various biological sources.
  • To assist researchers in selecting appropriate methods for EV preparation and characterization.
  • To highlight considerations for interpreting EV experimental data and address field limitations.

Main Methods:

  • Summarizes multi-step EV preparation processes.
  • Covers principles for performing and interpreting EV experiments.
  • Discusses analysis across diverse ecosystems, including body and environment sources.

Main Results:

  • Offers a comprehensive overview of EV analysis techniques.
  • Provides insights into method selection based on experimental needs.
  • Addresses challenges in EV research, including heterogeneity and environmental factors.

Conclusions:

  • Extracellular vesicle (EV) research is rapidly advancing, requiring careful method selection and interpretation.
  • Standardized approaches and clear guidelines are crucial for advancing EV applications in diagnostics and therapeutics.
  • This primer serves as a resource for navigating the complexities of EV analysis and research.