Consistent functional properties of MSC-exosomes from different batches revealed by comparative multi-omics,...

Area of Science:

• Cell biology
• Biotechnology
• Immunology

Background:

• Mesenchymal stromal cell-derived exosomes (MSC-EXOs) are investigated for anti-inflammatory properties and clinical applications.
• GMP-produced MSC-exosomes may differ in molecular content, necessitating source comparison.
• Adipose tissue-derived MSC-EXOs (ASC-EXOs) are a promising therapeutic candidate.

Purpose of the Study:

• To compare ASC-EXOs from three healthy donors isolated under Good Manufacturing Practice (GMP) conditions.
• To assess the consistency of ASC-EXO characteristics and therapeutic efficacy.
• To evaluate ASC-EXOs for potential clinical applications.

Main Methods:

• Exosomes characterized by nano-tracking analysis, cryo-electron microscopy, tetraspanin profiling, and multi-omics.
• Functional assays assessed anti-inflammatory effects and collagen production in vitro.
• In vivo efficacy evaluated in a mouse model of atopic dermatitis.

Main Results:

• No significant differences in ASC-EXO yield or characteristics were observed among batches.
• Multi-omics revealed minor cargo variations but highly similar functional profiles.
• ASC-EXOs demonstrated comparable in vitro anti-inflammatory and collagen-modulating effects and in vivo efficacy in an atopic dermatitis model.

Conclusions:

• ASC-EXOs from different batches exhibit consistent therapeutic effects.
• Biological efficacy and bioinformatics analysis are crucial for MSC-EXO quality control.
• Findings support the clinical development of ASC-EXOs.