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Related Experiment Video

Updated: Mar 24, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser capture microdissection.

Lance A Liotta¹, Thomas P Conrads², Marissa A Howard¹

¹Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.

Nature Reviews. Methods Primers

|March 23, 2026

Summary

This summary is machine-generated.

Laser capture microdissection (LCM) enables spatial molecular analysis by isolating specific tissue regions. This technology is crucial for understanding tissue heterogeneity, disease mechanisms, and developing future personalized therapies.

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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

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Last Updated: Mar 24, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Published on: February 6, 2015

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

The tissue microenvironment is a complex ecosystem studied at multiple resolution levels.
Spatial molecular interrogation tools are essential for understanding tissue complexity.

Purpose of the Study:

To highlight Laser capture microdissection (LCM) as a foundational technology for spatial molecular analysis.
To discuss LCM's applications in understanding tissue heterogeneity and disease mechanisms.
To explore the future of LCM in personalized medicine.

Main Methods:

Laser capture microdissection (LCM) physically isolates targeted cells or tissue compartments.
LCM enables downstream acquisition and analysis of nucleic acids, proteins, metabolites, and lipids.
LCM preserves spatial context for multiomic profiling.

Main Results:

LCM provides spatially resolved analysis of tissue heterogeneity and microenvironment interactions.
LCM is a versatile platform, agnostic to tissue type, molecular target, and analytical tool.
LCM is a key component in large-scale clinical trials for revealing disease mechanisms.

Conclusions:

LCM is a powerful tool for spatially resolved multiomic profiling.
LCM advances the understanding of disease mechanisms and therapeutic efficacy.
Next-generation LCM technologies will drive personalized and designer therapies.