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Updated: May 12, 2026

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
Published on: May 23, 2020
A practical guide to sample preparation for immunoprecipitation coupled with mass spectrometry analysis of C. elegans
Jian-Hua Wang1,2, Meng-Qiu Dong2,3
1College of Life Sciences, Capital Normal University, Beijing 100048, China.
Abstract:
The success of i mmuno p recipitation coupled with m ass s pectrometry (IPMS) analysis is tied to the amount of sample input and the expression level of the bait protein in the sample. Here, we selected six C. elegans proteins- VIT-2 , LMN-1 , ALG-1 , AAK-2 , DAF-16 , and PQM-1 -to represent high, medium, or low expression levels; each protein had a GFP tag knocked in at the endogenous gene locus. We varied the amount of input sample and analyzed the effect on IPMS identification results. This allowed us to offer an optimized IPMS protocol with the input sample amount adjusted according to the expression level of a bait protein.

